{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:20:18Z","timestamp":1761668418155,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,8,21]],"date-time":"2002-08-21T00:00:00Z","timestamp":1029888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In fly photoreceptor cells, light initiates a G protein\u2010coupled phospholipase C\u03b2\u2010dependent signaling cascade that results in the depolarization of the cell membrane, which is mediated by the cation channels TRP and TRPL. Together with phospholipase C\u03b2 and an eye\u2010specific protein kinase C, TRP is tethered to the scaffolding protein INAD, which forms a multimolecular signaling complex. Divergent data from expressed TRP and studies from photoreceptor cells have brought up a controversy whether or not a capacitative calcium entry (CCE) mechanism is involved in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    phototransduction pathway. Our initial characterization of TRP from photoreceptors of\n                    <jats:italic>Calliphora<\/jats:italic>\n                    vicina supported the hypothesis of a CCE mechanism, as heterologously expressed TRP was stimulated after application of thapsigargin. The situation changed when the PDZ domain protein INAD was coexpressed with TRP. In cells coexpressing TRP and INAD, no calcium entry was detectable on application of store depletion protocols. Suppression of CCE by INAD was not observed when the described interaction was disrupted by mutations in TRP and INAD. Our data show that apparent activation of TRP by CCE is abolished by INAD. Within the complex, the proteins necessary for phototransduction mutually influence their activities. The results support the hypothesis of a store\u2010independent activation of TRP.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0192fje","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T19:22:27Z","timestamp":1034191347000},"page":"1668-1670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The PDZ\u2010scaffold protein INAD abolishes apparent store\u2010dependent regulation of the light\u2010activated cation channel TRP"],"prefix":"10.1096","volume":"16","author":[{"given":"Christian","family":"Harteneck","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pharmakologie Universit\u00e4tsklinikum Benjamin Franklin, Freie Universit\u00e4t Berlin  Thielallee 69\u201373 14195 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabina Naranjo","family":"Kuchta","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie Universit\u00e4tsklinikum Benjamin Franklin, Freie Universit\u00e4t Berlin  Thielallee 69\u201373 14195 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Huber","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Zoologie, Abteilung Zellbiologie und Neurobiologie Universit\u00e4t Karlsruhe  Haid\u2010und\u2010Neu\u2010Stra\u00dfe 9 76131 Karlsruhe Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Paulsen","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Zoologie, Abteilung Zellbiologie und Neurobiologie Universit\u00e4t Karlsruhe  Haid\u2010und\u2010Neu\u2010Stra\u00dfe 9 76131 Karlsruhe Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"Schultz","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie Universit\u00e4tsklinikum Benjamin Franklin, Freie Universit\u00e4t Berlin  Thielallee 69\u201373 14195 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,8,21]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"2340","article-title":"Drosophila in vision research","volume":"36","author":"Pak W. 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