{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:25:09Z","timestamp":1792193109294,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p>\n                    Intratracheal instillation of lipopolysaccharide (LPS) activates alveolar macrophages and infiltration of neutrophils, causing lung injury\/acute respiratory distress syndrome. Free radicals are a special focus as the final causative molecules in the pathogenesis of lung injury caused by LPS. Although in vitro investigation has demonstrated radical generation after exposure of cells to LPS,\n                    <jats:italic>in vivo<\/jats:italic>\n                    evidence is lacking. Using electron spin resonance (ESR) and the spin trap a\u2010(4\u2010pyridyl\u20101\u2010oxide)\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010tert\u2010butylnitrone (POBN), we investigated\n                    <jats:italic>in vivo<\/jats:italic>\n                    free radical production by rats treated with intratracheal instillation of LPS. ESR spectroscopy of lipid extract from lungs exposed to LPS for 6 h gave a spectrum consistent with that of a POBN\/ carbon\u2010centered radical adduct (\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sup>N<\/jats:sup>\n                    =14.94\u00b10.07 G and\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>:<\/jats:sub>\n                    <jats:sup>H<\/jats:sup>\n                    =2.42\u00b10.06 G) tentatively assigned as a product of lipid peroxidation. To further investigate the mechanism of LPS\u2010initiated free radical generation, rats were pretreated with the phagocytic toxicant GdCl\n                    <jats:sub>3<\/jats:sub>\n                    , which significantly decreased the production of radical ad\u2010ducts with a Corresponding decrease in neutrophil infiltration. NADPH oxidase knockout mice completely blocked phagocyte\u2010mediated, ESR\u2010detectable radical production in this model of acute lung injury. Rats treated intratracheally with LPS generate lipid\u2010derived free radicals via activation of NADPH oxidase.\u2014Sato, K., Kadiiska, M. B., Ghio, A. J., Corbett, J., Fann, Y. C., Holland, S. M., Thurman, R. G., Mason, R. P. In vivo lipid\u2010derived free radical formation by NADPH oxidase in acute lung injury induced by lipopolysaccharide: a model for ARDS.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 16, 1713\u20131720 (2002)\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0331com","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T13:44:20Z","timestamp":1035985460000},"page":"1713-1720","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":144,"title":["In vivo lipid\u2010derived free radical formation by NADPH oxidase in acute lung injury induced by lipopolysaccharide: a model for ARDS"],"prefix":"10.1096","volume":"16","author":[{"given":"Keizo","family":"Sato","sequence":"first","affiliation":[{"name":"Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry National Institute of Environmental Health Sciences, National Institutes of Health  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria B.","family":"Kadiiska","sequence":"additional","affiliation":[{"name":"Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry National Institute of Environmental Health Sciences, National Institutes of Health  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Ghio","sequence":"additional","affiliation":[{"name":"National Health and Environmental Effects Research Laboratory Environmental Protection Agency  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Corbett","sequence":"additional","affiliation":[{"name":"Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry National Institute of Environmental Health Sciences, National Institutes of Health  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang C.","family":"Fann","sequence":"additional","affiliation":[{"name":"Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry National Institute of Environmental Health Sciences, National Institutes of Health  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Holland","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald G.","family":"Thurman","sequence":"additional","affiliation":[{"name":"Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, and Curriculum in Toxicology University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald P.","family":"Mason","sequence":"additional","affiliation":[{"name":"Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry National Institute of Environmental Health Sciences, National Institutes of Health  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"913","article-title":"Endotoxin and lung injury","volume":"133","author":"Brigham K. 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