{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T22:53:57Z","timestamp":1781218437805,"version":"3.54.1"},"reference-count":61,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2003,3,5]],"date-time":"2003-03-05T00:00:00Z","timestamp":1046822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The effects of neonatal dexamethasone (DEX) treatment on spatial learning and hippocampal synaptic plasticity were investigated in adult rats. Spatial learning in reference and working memory versions of the Morris maze was impaired in DEX\u2010treated rats. In hippocampal slices of DEX rats, long\u2010term depression was facilitated and potentiation was impaired. Paired\u2010pulse facilitation was normal, suggesting a postsynaptic defect as cause of the learning and plasticity deficits. Western blot analysis of hippocampal postsynaptic densities (PSD) revealed a reduction in NR2B subunit protein, whereas the abundance of the other major\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010aspartate (NMDA) receptor subunits (NR1, NR2A), AMPA receptor subunits (GluR2\/3), scaffolding proteins, and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \/calmodulin\u2010dependent protein kinase II (\u03b1CaMKII) were unaltered. This selective reduction in NR2B likely resulted from altered receptor assembly rather than subunit expression, because the abundance of NR2B in the homogenate and crude synaptosomal fractions was unaltered. In addition, the activity of \u03b1CaMKII, an NMDA receptor complex associated protein kinase, was increased in PSD of DEX rats. The results indicate that neonatal treatment with DEX causes alterations in composition and function of the hippocampal NMDA receptor complex that persist into adulthood. These alterations likely explain the deficits in hippocampal synaptic plasticity and spatial learning induced by neonatal DEX treatment.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0333fje","type":"journal-article","created":{"date-parts":[[2003,4,30]],"date-time":"2003-04-30T16:03:40Z","timestamp":1051718620000},"page":"1-22","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Long\u2010lasting effects of neonatal dexamethasone treatment on spatial learning and hippocampal synaptic plasticity. Involvement of the NMDA receptor complex"],"prefix":"10.1096","volume":"17","author":[{"given":"Patrick J. G. H.","family":"Kamphuis","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Anatomy Rudolf Magnus Institute of Neuroscience University Medical Center Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Gardoni","sequence":"additional","affiliation":[{"name":"Department Pharmacological Sciences University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amer","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Anatomy Rudolf Magnus Institute of Neuroscience University Medical Center Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerda","family":"Croiset","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Anatomy Rudolf Magnus Institute of Neuroscience University Medical Center Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost M.","family":"Bakker","sequence":"additional","affiliation":[{"name":"Department of Neonatology Wilhelmina Children's Hospital University Medical Center Utrecht  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flaminio","family":"Cattabeni","sequence":"additional","affiliation":[{"name":"Department Pharmacological Sciences University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem Hendrik","family":"Gispen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Anatomy Rudolf Magnus Institute of Neuroscience University Medical Center Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Van Bel","sequence":"additional","affiliation":[{"name":"Department of Neonatology Wilhelmina Children's Hospital University Medical Center Utrecht  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Di Luca","sequence":"additional","affiliation":[{"name":"Department Pharmacological Sciences University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M.","family":"Wiegant","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Anatomy Rudolf Magnus Institute of Neuroscience University Medical Center Utrecht"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198906083202301"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.319.7222.1385"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2000.107799"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2431-1-1","article-title":"The adverse neuro-developmental effects of postnatal steroids in the preterm infant: a systematic review of RCTs","volume":"1","author":"Barrington K. 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