{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T22:24:29Z","timestamp":1791671069781,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL69029-01"],"award-info":[{"award-number":["HL69029-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA89366"],"award-info":[{"award-number":["CA89366"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p>\n                    There is growing evidence for the involvement of nitric oxide (NO) \u2010mediated nitrosation in cell signaling and pathology. Although S\u2010nitrosothiols (RSNOs) have been frequently implicated in these processes, it is unclear whether NO forms nitrosyl adducts with moieties other than thiols. A major obstacle in assessing the significance of formation of nitro\u2010sated species is the limited reliability of available analytical techniques for measurements in complex biological matrices. Here we report on the presence of nitrosated compounds in plasma and erythrocytes of rats, mice, guinea pigs, and monkeys under basal conditions, in immunologically challenged murine macrophages in vitro and laboratory animals in vivo. Besides RSNOs, all biological samples also contained mercury\u2010stable nitroso species, indicating the additional involvement of amine and heme nitros(yl)ation reactions. Significant differences in the amounts and ratios of RSNOs over N\u2010and heme\u2010nitros(yl)ated compounds were found between species and organs. These observations were made possible by the development of a novel gas\u2010phase chemiluminescence\u2010based technique that allows detection of nitroso species in tissues and biological fluids without prior extraction or deproteinization. The method can quantify as little as 100 fmol bound NO and has been validated extensively for use in different biological matrices. Discrimination between nitrite, RSNOs, and N\u2010nitroso or nitrosylheme compounds is accomplished by use of group\u2010specific reagents. Our findings suggest that NO generation in vivo leads to concomitant formation of RSNOs, nitrosamines, and nitrosylhemes with considerable variation between rodents and primates, highlighting the difficulty in comparing data between different animal models and extrapolating results from experimental animals to human physiology.\u2014Feelisch, M., Rassaf, T., Mnaimneh, S., Singh, N., Bryan, N. S., Jourd'heuil, D., Kelm, M. Concomitant S\u2010, N\u2010, and heme\u2010nitros(yl)ation in biological tissues and fluids: implications for the fate of NO in vivo.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 16, 1775\u20131785 (2002)\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0363com","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T13:44:20Z","timestamp":1035985460000},"page":"1775-1785","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":346,"title":["Concomitant S\u2010, N\u2010, and heme\u2010nitros(yl)ation in biological tissues and fluids: implications for the fate of NO in vivo"],"prefix":"10.1096","volume":"16","author":[{"given":"Martin","family":"Feelisch","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Physiology LSU Health Sciences Centers  Shreveport Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tienush","family":"Rassaf","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology LSU Health Sciences Centers  Shreveport Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanie","family":"Mnaimneh","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology LSU Health Sciences Centers  Shreveport Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology LSU Health Sciences Centers  Shreveport Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan S.","family":"Bryan","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology LSU Health Sciences Centers  Shreveport Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Jourd'Heuil","sequence":"additional","affiliation":[{"name":"Center for Cardiovascular Sciences Albany Medical College  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malte","family":"Kelm","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiology, Pulmonary Diseases and Angiology Heinrich\u2010Heine\u2010University  D\u201040225 Duesseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"7","volume-title":"Handbook of Experimental Pharmacology","author":"Wink D. 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