{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:50:51Z","timestamp":1790920251532,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["AI 31268"],"award-info":[{"award-number":["AI 31268"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["AI 46029"],"award-info":[{"award-number":["AI 46029"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The immunoregulatory neuropeptide vasoactive intestinal peptide (VIP) was cleaved by purified IgG from\n                    <jats:italic>Fas<\/jats:italic>\n                    \u2010defective C3H\/\n                    <jats:italic>gld<\/jats:italic>\n                    mice, lupus patients, and autoimmune thyroiditis patients. No VIPase activity was detected in IgG from control mice and humans. Kinetic analyses of VIPase IgG preparations suggested low\u2010affinity recognition of VIP. Yet the VIPase activity was VIP selective, judged by lack of correlation with other protease activities expressed by the IgG and by noninterference of unrelated peptides in the activity. Recombinant Fv constructs selected from a human lupus phage show library displayed VIPase activity, confirming that the active site is located in the V domains. Inhibition of the VIPase activity by di\u2010isopropylfluorophosphate suggested a serine protease\u2010like mechanism of catalysis. Irreversible binding of a biotinyated phosphonate diester by the IgG and Fv preparations was observed, consistent with the presence of activated nucleophiles similar to those in enzymes capable of covalent catalysis. These observations show that VIP is a target for specific catalytic autoantibodies in autoimmune disease.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0475com","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T21:03:22Z","timestamp":1048885402000},"page":"628-635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["VIPase autoantibodies in\n                    <i>Fas<\/i>\n                    \u2010defective mice and patients with autoimmune disease"],"prefix":"10.1096","volume":"17","author":[{"given":"Yogesh","family":"Bangale","sequence":"first","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangeeta","family":"Karle","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Planque","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Xin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Li","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravishankar","family":"Kalaga","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhir","family":"Paul","sequence":"additional","affiliation":[{"name":"Chemical Immunology Research Center Departments of Pathology and Medicine University of Texas Medical School Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03348097"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/pupt.2001.0337"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-5428(96)00007-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb06953.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.2.1028"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1159\/000097344","article-title":"Direct morphological evidence of neuroimmunomodulation in colonic mucosa of patients with Crohn's disease","volume":"4","author":"Feher E.","year":"1997","journal-title":"Neuroimmunomodulation"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00342-5"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241503798"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0671com"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/87887"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1585181"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.3.1530"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2425"},{"key":"e_1_2_6_15_1","first-page":"587","article-title":"Catalytic cleavage of vasopressin by human Bence Jones proteins at the arginylglycinamide bond","volume":"377","author":"Matsuura K.","year":"1996","journal-title":"Biol. 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