{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:50:28Z","timestamp":1777589428928,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,1,22]],"date-time":"2003-01-22T00:00:00Z","timestamp":1043193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:p>\n                    An \u03b1\u2010toxin responsible for nitric oxide (NO) release in rabbit corpus cavernosum (RbCC) was isolated from\n                    <jats:italic>Tityus serrulatus<\/jats:italic>\n                    venom (TSV). The isolated peptide (molecular mass of 7427.66\u00b10.15 Da) was identified as Ts3 after determination of Cys residues, N\u2010terminal amino acid analysis, and proteolytic peptide mapping. Ts3 (30 nM) markedly relaxed the RbCC; this response was blocked by the NO synthesis inhibitor N\n                    <jats:sup>\u03c9<\/jats:sup>\n                    \u2010nitro\u2010\n                    <jats:sc>L<\/jats:sc>\n                    \u2010arginine methyl ester (100 \u03bcM) and the Na\n                    <jats:sup>+<\/jats:sup>\n                    channel blocker tetrodotoxin (100 nM). Synthetic peptides based on either Ts3 (P\n                    <jats:sup>1\u201316<\/jats:sup>\n                    , p\n                    <jats:sup>17\u201332<\/jats:sup>\n                    , p\n                    <jats:sup>33\u201348<\/jats:sup>\n                    , p\n                    <jats:sup>49\u201364<\/jats:sup>\n                    , p\n                    <jats:sup>9\u201324<\/jats:sup>\n                    , p\n                    <jats:sup>25\u201340<\/jats:sup>\n                    , p\n                    <jats:sup>41\u201356<\/jats:sup>\n                    , YGLPDKVPTKT) or Bukatoxin (isolated from\n                    <jats:italic>Buthus martensi<\/jats:italic>\n                    Karsch scorpion venom) sequence (Buka11, Buka11\u2010B, PDKVP, PDSEP) were assayed. These peptides slightly relaxed the RbCC, and such an effect was independent of Na\n                    <jats:sup>+<\/jats:sup>\n                    channel activation or NO release. Our results indicate that Ts3 exerts nitrergic actions and contributes to the relaxing activity of TSV in RbCC, thus providing a valuable tool to investigate the mechanisms underlying nerve activation in erectile tissues, because NO released from nitrergic fibers plays a key role in the erectile process. Our findings revealed the key importance of the Ts3 structure three\u2010dimensional conformation maintenance for biological activity, because linear peptide sequences neither presented substantial relaxations nor was this effect related to nitrergic activity.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0635fje","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:42:07Z","timestamp":1047404527000},"page":"1-17","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Sequence and structure\u2010activity relationship of a scorpion venom toxin with nitrergic activity in rabbit corpus cavernosum"],"prefix":"10.1096","volume":"17","author":[{"given":"Cleber E.","family":"Teixeira","sequence":"first","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences UNICAMP Campinas Sao Paulo 13081\u2010970"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demian R.","family":"Ifa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Institute of Biomedical Sciences USP Sao Paulo 05508\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaetano","family":"Corsoi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Biotechnology University of Naples Federico II Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Santagada","sequence":"additional","affiliation":[{"name":"Department of Toxicological and Pharmaceutical Chemistry University of Naples Federico II Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Caliendo","sequence":"additional","affiliation":[{"name":"Department of Toxicological and Pharmaceutical Chemistry University of Naples Federico II Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences UNICAMP Campinas Sao Paulo 13081\u2010970"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"De Nucci","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences UNICAMP Campinas Sao Paulo 13081\u2010970"},{"name":"Department of Pharmacology Institute of Biomedical Sciences USP Sao Paulo 05508\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,1,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00625.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(99)01398-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00203a010"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569549809009247"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(98)00111-7"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01167-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(80)80082-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582058"},{"key":"e_1_2_6_10_1","first-page":"683","volume-title":"Handbook of Neurotoxicology","author":"Martin\u2010Eauclaire M. 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