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We present several lines of evidence that DChol mimics cholesterol. The cholesterol probe could be efficiently incorporated into the plasma membrane via cyclodextrin\u2010donor complexes. The itinerary of DChol from the plasma membrane to the cell was studied to determine its dependence on the function of Niemann\u2010Pick C1 (NPC) protein. In all cells, DChol moved from the plasma membrane to the endoplasmic reticulum. Its further transport to the Golgi complex was observed but with marked differences among various cell lines. DChol was finally transported to small (\u223c0.5 \u00b5m diameter) lipid droplets, a process that required functional acyl\u2010CoA:cholesterol acyltransferase. In human NPC fibroblasts, NPC\u2010like cells, or in cells mimicking the NPC phenotype, DChol was found in enlarged (&gt;1 \u00b5m diameter) droplets. When the NPC\u2010phenotype was corrected by transfection with NPC1, DChol was again found in small\u2010sized droplets. Our data show that NPC1 has an essential role in the distribution of plasma membrane\u2010derived cholesterol by maintaining the small size of cholesterol\u2010containing lipid droplets in the cell.<\/jats:p>","DOI":"10.1096\/fj.02-0818fje","type":"journal-article","created":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T21:03:22Z","timestamp":1048885402000},"page":"782-784","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Transport of plasma membrane\u2010derived cholesterol and the function of Niemann\u2010Pick C1 protein"],"prefix":"10.1096","volume":"17","author":[{"given":"Volker","family":"Wiegand","sequence":"first","affiliation":[{"name":"Institute of Biochemistry Johannes Gutenberg\u2010University of Mainz Becherweg 30 D\u201055099 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u2010Yuan","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of 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