{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:29:48Z","timestamp":1791307788860,"version":"4.3.1"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2003,7,3]],"date-time":"2003-07-03T00:00:00Z","timestamp":1057190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["BIL 00\/36"],"award-info":[{"award-number":["BIL 00\/36"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The inhibitor cystine knot (ICK) fold is an evolutionarily conserved structural motif shared by a large group of polypeptides with diverse sequences and bioactivities. Although found in different phyla (animal, plant, and fungus), ICK peptides appear to be most prominent in venoms of cone snail and spider. Recently, two scorpion toxins activating a calcium release channel have been found to adopt an ICK fold. We have isolated and identified both cDNA and genomic clones for this family of ICK peptides from the scorpion\n                    <jats:italic>Opistophthalmus carinatus<\/jats:italic>\n                    . The gene characterized by three well\u2010delineated exons respectively coding for three structural and functional domains in the toxin precursors illustrates the correlation between exon and module as suggested by the \u201cexon theory of genes.\u201d Based on the analysis of precursor organization and gene structure combined with the 3\u2010D fold and functional data, our results highlight a common evolutionary origin for ICK peptides from animals. In contrast, ICK peptides from plant and fungus might be independently evolved from another ancestor.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-1044fje","type":"journal-article","created":{"date-parts":[[2003,7,29]],"date-time":"2003-07-29T16:11:57Z","timestamp":1059495117000},"page":"1765-1767","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":136,"title":["Evolutionary origin of inhibitor cystine knot peptides"],"prefix":"10.1096","volume":"17","author":[{"given":"Shunyi","family":"Zhu","sequence":"first","affiliation":[{"name":"Laboratory of Toxicology, University of Leuven  E. Van Evenstraat 4 3000 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herve","family":"Darbon","sequence":"additional","affiliation":[{"name":"AFMB CNRS UMR 6098, et Universit\u00e9s d'Aix-Marseille I and II  31 Chemin Joseph Aiguier 13402 Marseille Cedex 20 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Dyason","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Potchefstroom  Private Bag X6001 Potchefstroom 2520 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fons","family":"Verdonck","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Centre University of Leuven Campus Kortrijk  8500 Kortrijk Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology, University of Leuven  E. 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