{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:55:19Z","timestamp":1762160119070,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,1]],"date-time":"2004-02-01T00:00:00Z","timestamp":1075593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006831","name":"U.S. Air Force","doi-asserted-by":"publisher","award":["#F49620\u201000\u20101\u20100349"],"award-info":[{"award-number":["#F49620\u201000\u20101\u20100349"]}],"id":[{"id":"10.13039\/100006831","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>\n                    We define five unique cellular responses to thermal stress using a reporter construct generated using the stress\u2010inducible promoter from the gene encoding a murine 70 kDa heat shock protein (Hsp70A.1) to express luciferase (\n                    <jats:italic>luc<\/jats:italic>\n                    ). Thermal stress was delivered over a range of temperatures (42\u201368\u00b0C) for5sto20min and luciferase activity was measured in live cells using a cooled CCD camera as a measure of reporter gene transcription. Reporter gene expression was assessed every 2 h for 10 h, and at 24 h post\u2010stress. Expression patterns were validated for selected tem\u00acperatures. A transition zone where cells lose the ability to produce light and beyond which &gt;50% of cells die was observed to occur within a narrow (2.5\u00b0C) temper\u00acature window. Although\n                    <jats:italic>luc<\/jats:italic>\n                    and\n                    <jats:italic>hsp70<\/jats:italic>\n                    mRNA levels in this transition zone were high, there were reduced levels of Luc and Hsp70 protein and ATP levels. Cells treated at these temperatures recovered the ability to produce light in response to a secondary stress at 30 h. This Hsp70\u2010luc reporter gene construct may be useful for defining zones of physiologic responses and assess\u00acing collateral thermal damage generated during treat\u00acment of biological tissue with lasers and other sources of heat.\u2014O'Connell\u2010Rodwell, C. E., Shriver, D., Simanovskii, D. M., McClure, C., Cao, Y\u2010a., Zhang, W., Bachmann, M. H., Beckham, J. T., Jansen, E. D., Palanker, D., Schwettman, H. A., Contag, C. H. A genetic reporter of thermal stress defines physiologic zones over a defined temperature range.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    18, 264\u2013271 (2004)\n                  <\/jats:p>","DOI":"10.1096\/fj.03-0585com","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T19:38:02Z","timestamp":1076355482000},"page":"264-271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["A genetic reporter of thermal stress defines physiologic zones over a defined temperature range"],"prefix":"10.1096","volume":"18","author":[{"given":"Caitlin E.","family":"O'connell\u2010Rodwell","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Microbiology &amp; Immunology and Radiology Stanford School of Medicine Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Shriver","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Microbiology &amp; Immunology and Radiology Stanford School of Medicine Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitri M.","family":"Simanovskii","sequence":"additional","affiliation":[{"name":"Picosecond Free Electron Laser Center, W. 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