{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:15:33Z","timestamp":1789193733571,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T00:00:00Z","timestamp":1173139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["0120648"],"award-info":[{"award-number":["0120648"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009193","name":"Marsden Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009193","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The \u201cEquilibrium Model\u201d has provided new tools for describing and investigating enzyme thermal adaptation. It has been shown that the effect of temperature on enzyme activity is not only governed by \u2206\n                    <jats:italic>G<\/jats:italic>\n                    <jats:sup>\u2021<\/jats:sup>\n                    <jats:sub>cat<\/jats:sub>\n                    and \u2206\n                    <jats:italic>G<\/jats:italic>\n                    <jats:sup>\u2021<\/jats:sup>\n                    <jats:sub>inact<\/jats:sub>\n                    but also by two new intrinsic parameters, \u2206\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    , which describe the enthalpy and midpoint, respectively, of a reversible equilibrium between active and inactive (but not denatured) forms of enzyme. Twenty\u2010one enzymes from organisms with a wide range of growth temperatures were characterized using the Equilibrium Model. Statistical analysis indicates that\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    is a better predictor of growth temperature than enzyme stability (\u2206\n                    <jats:italic>G<\/jats:italic>\n                    <jats:sup>\u2021<\/jats:sup>\n                    <jats:sub>inact<\/jats:sub>\n                    ). As expected from the Equilibrium Model, \u2206\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    correlates with catalytic temperature tolerance of enzymes and thus can be declared the first intrinsic and quantitative measure of enzyme eurythermalism. Other findings shed light on the evolution of psychrophilic and thermophilic enzymes. The findings suggest that the description of the Equilibrium Model of the effect of temperature on enzyme activity applies to all enzymes regardless of their temperature origins and that its associated parameters, \u2206\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    and\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>eq<\/jats:sub>\n                    , are intrinsic and necessary parameters for characterizing the thermal properties of enzymes and their temperature adaptation and evolution.\u2014Lee C. K., Daniel, R. M., Shepherd, C., Saul, D., Cary, S. C., Danson, M. J., Eisenthal, R., Peterson M. E. Eurythermal\u2010ism and the temperature dependence of enzyme activity\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    21, 1934\u20131941 (2007)\n                  <\/jats:p>","DOI":"10.1096\/fj.06-7265com","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T22:25:26Z","timestamp":1173219926000},"page":"1934-1941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Eurythermalism and the temperature dependence of enzyme activity"],"prefix":"10.1096","volume":"21","author":[{"given":"Charles K.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biological Sciences University of Waikato Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy M.","family":"Daniel","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Waikato Hamilton New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charis","family":"Shepherd","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Saul","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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