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LGALS15 contains a predicted carbohydrate recognition domain (CRD) as well as LDV and RGD recognition sequences for integrin binding. Studies tested the hypothesis that LGALS15 is a secreted regulator of blastocyst development, as well as growth, migration, adhesion, and apoptosis of tro\u2010phoblast. Bovine embryos were produced\n                    <jats:italic>in vitro<\/jats:italic>\n                    by standard conditions, and putative zygotes were cultured in the presence of recombinant ovine LGALS15. Rates of embryo cleavage and blastocyst formation were not affected by LGALS15. LGALS15 moderately increased proliferation of ovine trophectoderm (oTr) cells. Staurosporine elicited apoptosis of oTr cells, which could be partially inhibited by LGALS15. Migration of oTr cells was stimulated by LGALS15 that was dependent on Jun N\u2010terminal kinase (JNK). A dose\u2010dependent increase in oTr cell attachment to LGALS15 was found that could be inhibited by cyclic GRGDS, but not GRADS, peptides. Mutation of the LDVRGD integrin binding sequence of LGALS15 to LADRAD decreased its ability to promote oTr cell attachment, whereas mutation of the CRD had little effect. LGALS15 induced formation of robust focal adhesions in oTr cells that was abolished by mutation of the LDVRGD sequence. Collectively, these results support the hypothesis that LGALS15 stimulates trophectoderm cell migration and attachment\n                    <jats:italic>via<\/jats:italic>\n                    integrin binding and activation which are critical to blastocyst elongation and implantation.\u2014Farmer, J. L., Burghardt, R. C., Jousan, F. D., Hansen, P. J., Bazer, F. W., Spencer, T. E. Galectin 15 (LGALS15) functions in trophectoderm migration and attachment.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    22, 548\u2013560 (2008)\n                  <\/jats:p>","DOI":"10.1096\/fj.07-9308com","type":"journal-article","created":{"date-parts":[[2007,9,21]],"date-time":"2007-09-21T20:44:27Z","timestamp":1190407467000},"page":"548-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Galectin 15 (LGALS15) functions in trophectoderm migration and attachment"],"prefix":"10.1096","volume":"22","author":[{"given":"Jennifer L.","family":"Farmer","sequence":"first","affiliation":[{"name":"Center for Animal Biotechnology and Genomics and Department of Animal Science Texas A&amp;M University College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Burghardt","sequence":"additional","affiliation":[{"name":"Image Analysis Laboratory and Department of Veterinary Integrative Biosciences Texas A&amp;M University College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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