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Neutral amino acids are generated by a variety of aminopeptidases and carboxypeptidases and are subsequently taken up by the amino acid transporter B\n                    <jats:sup>0<\/jats:sup>\n                    AT1 (SLC6A19), which is mutated in Hartnup disorder. Coexpression of B\n                    <jats:sup>0<\/jats:sup>\n                    AT1 together with the brush\u2010border carboxypeptidase angiotensin\u2010converting enzyme 2 (ACE2) in\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    oocytes led to a dramatic increase of transporter expression at the oocyte surface. Other members of the SLC6 family were not stimulated by coexpression with ACE2. Addition of a peptide containing a carboxyterminal leucine residue to ACE2\u2010 and B\n                    <jats:sup>0<\/jats:sup>\n                    AT1\u2010coexpressing oocytes caused inward currents due to Na\n                    <jats:sup>+<\/jats:sup>\n                    \u2010leucine cotransport, demonstrating the formation of a metabolic complex. Coexpression of the Hartnup disorder causing mutation B\n                    <jats:sup>0<\/jats:sup>\n                    AT1(R240Q) showed reduced interaction with ACE2 and its renal paralogue collectrin. This would result in reduced surface expression in both kidney and intestine, thereby explaining the onset of the disorder in individuals carrying this mutation.\u2014Kowalczuk, S., Br\u00f6er, A., Tietze, N., Vanslambrouck, J. M., Rasko, J. E. J., Br\u00f6er, S. A protein complex in the brush\u2010border membrane explains a Hartnup disorder allele.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    22, 2880\u20132887 (2008)\n                  <\/jats:p>","DOI":"10.1096\/fj.08-107300","type":"journal-article","created":{"date-parts":[[2008,4,18]],"date-time":"2008-04-18T21:36:30Z","timestamp":1208554590000},"page":"2880-2887","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":197,"title":["A protein complex in the brush\u2010border membrane explains a Hartnup disorder allele"],"prefix":"10.1096","volume":"22","author":[{"given":"Sonja","family":"Kowalczuk","sequence":"first","affiliation":[{"name":"School of Biochemistry and Molecular Biology Australian National University Canberra Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Br\u00f6er","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Molecular Biology Australian National University Canberra Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Tietze","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Molecular Biology Australian National University Canberra Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Vanslambrouck","sequence":"additional","affiliation":[{"name":"Gene and Stem Cell Therapy Program Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney Camperdown Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E. 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