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It remains controversial as to the mode (antiparallel or parallel) of \u03b1\u2010S self\u2010assembly and whether an exact alignment of the central hydrophobic region is essential. In the present study, we performed\n                    <jats:italic>in vitro<\/jats:italic>\n                    assembly using \u03b1\u2010S with or without the attachment of artificial leucine zippers (Zips) capable of forming either parallel or antiparallel coiled coils and included a spacer in one derivative. Results showed that Zips accelerate filament assembly in both the parallel and antiparallel fashions, that a precise alignment of the central hydrophobic region is not essential, and that the antiparallel pairs displayed the highest thioflavin T signals. More importantly, cells expressing Zip\u2010fused \u03b1\u2010S, but not \u03b1\u2010S alone, formed \u03b1\u2010S immunopositive and thioflavin S\u2010positive inclusions in 7 days. The results suggest that \u03b1\u2010S can assemble in both parallel and antiparallel modes but have a higher tendency to assemble in the latter mode and that cells overexpressing Zip\u2010fused \u03b1\u2010S may be used to screen \u03b1\u2010S assembly inhibitors due to enhanced ability to form inclusions.\u2014Jiang, P., Ko, L., Jansen, K. R., Golde, T. E., Yen, S.\u2010H. Using leucine zipper to facilitate \u03b1\u2010synuclein assembly.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    22, 3165\u20133174 (2008)\n                  <\/jats:p>","DOI":"10.1096\/fj.08-108365","type":"journal-article","created":{"date-parts":[[2008,5,20]],"date-time":"2008-05-20T23:08:49Z","timestamp":1211324929000},"page":"3165-3174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Using leucine zipper to facilitate \u03b1\u2010synuclein assembly"],"prefix":"10.1096","volume":"22","author":[{"given":"Peizhou","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Neuroscience Mayo Clinic College of Medicine  Jacksonville Florida USA"},{"name":"Department of Histology and Embryology School of Basic Medical Science Southern Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010wen","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Mayo Clinic College of Medicine  Jacksonville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen R.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Mayo Clinic College of Medicine  Jacksonville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd E.","family":"Golde","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Mayo Clinic College of Medicine  Jacksonville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010Hui","family":"Yen","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Mayo Clinic College of Medicine  Jacksonville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,5,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90302-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-08-02804.1988"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007020050002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.58.2.186"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.11282"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961799n"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.09.001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008919200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/3311"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01419-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00514-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi991447r"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.14.9843"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi052344d"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5422com"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700368200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305266200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507405102"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3289117"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2911757"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1948029"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340070102"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981269m"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(96)10052-9"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.5.2583"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5238.935"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi962391t"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3352"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/10897"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"8237","DOI":"10.1021\/ja020275+","article-title":"A general method for selection and screening of coiled coils on the basis of relative helix orientation","volume":"124","author":"Kim B. 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