{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:26:17Z","timestamp":1791048377707,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,22]],"date-time":"2008-12-22T00:00:00Z","timestamp":1229904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Plasma membrane chloride (C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) pathways play an important role in neuronal physiology. Here, we investigated the role of NKCC1 cotransporters (a secondary active C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    uptake mechanism) in C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    handling in cultured rat dorsal root ganglion neurons (DRGNs) and motor neurons (MNs) derived from fetal stage embryonic day 14. Gramicidin\u2010perforated patch\u2010clamp recordings revealed that DRGNs accumulate intracellular C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    through a bumetanide\u2010 and Na\n                    <jats:sup>+<\/jats:sup>\n                    \u2010sensitive mechanism, indicative of the functional expression of NKCC1. Western blotting confirmed the expression of NKCC1 in both DRGNs and MNs, but immunocytochemistry experiments showed a restricted expression in dendrites of MNs, which contrasts with a homogeneous expression in DRGNs. Both MNs and DRGNs could be readily loaded with or depleted of CU during GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor activation at depolarizing or hyperpolarizing membrane potentials. After loading, the rate of recovery to the resting CU concentration (\n                    <jats:italic>i.e<\/jats:italic>\n                    ., [C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    decrease) was similar in both cell types and was unaffected by lowering the extracellular Na\n                    <jats:sup>+<\/jats:sup>\n                    concentration. In contrast, the recovery on depletion (\n                    <jats:italic>i.e<\/jats:italic>\n                    ., [C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    increase) was significantly faster in DRGNs in control conditions but not in low extracellular Na\n                    <jats:sup>+<\/jats:sup>\n                    . The experimental observations could be reproduced by a mathematical model for intracellular CU kinetics, in which DRGNs show higher NKCC1 activity and smaller C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2010handling volume than MNs. On the basis of these results, we conclude that embryonic DRGNs show a higher somatic functional expression of NKCC1 than embryonic MNs. The high NKCC1 activity in DRGNs is important for maintaining high [C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    , whereas lower NKCC1 activity in MNs allows large [C1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    variations during neuronal activity.\u2014Chabwine, J. N., Talavera, K., Verbert, L., Eggermont, J., Vanderwinden, J.\u2010M., De Smedt, H., Van Den Bosch, L., Robberecht, W., Callewaert, G. Differential contribution of the Na+\u2010cK+\u20102Cl\u2212 cotransporter NKCC1 to chloride handling in rat embryonic dorsal root ganglion neurons and motor neurons.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 23, 1168\u20131176 (2009)\n                  <\/jats:p>","DOI":"10.1096\/fj.08-116012","type":"journal-article","created":{"date-parts":[[2008,12,22]],"date-time":"2008-12-22T20:26:01Z","timestamp":1229977561000},"page":"1168-1176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Differential contribution of the Na\n                    <sup>+<\/sup>\n                    \u2010K\n                    <sup>+<\/sup>\n                    \u20102Cl\n                    <sup>\u2212<\/sup>\n                    cotransporter NKCC1 to chloride handling in rat embryonic dorsal root ganglion neurons and motor neurons"],"prefix":"10.1096","volume":"23","author":[{"given":"J. N.","family":"Chabwine","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Talavera","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Verbert","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Eggermont","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-M.","family":"Vanderwinden","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology Universit\u00e9 Libre de Bruxelles Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"De Smedt","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Van Den Bosch","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Robberecht","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Callewaert","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Katholieke Universiteit Leuven, Vesalius Research Center, VIB Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002210050637"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-12-04942.2003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002210050933"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2003.12.015"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1301"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.64.081501.155847"},{"key":"e_1_2_7_8_1","first-page":"309","article-title":"Cation-chloride cotransporters in neuronal communication","volume":"15","author":"Delpire E.","year":"2000","journal-title":"News Physiol. 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