{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:15:00Z","timestamp":1791947700556,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T00:00:00Z","timestamp":1229040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dual oxidases (Duoxl and Duox2) are plasma membrane\u2010targeted hydrogen peroxide generators that support extracellular hemoperoxidases. Duox activator 2 (Duoxa2), initially described as an endoplasmic reticulum resident protein, functions as a maturation factor needed to deliver active Duox2 to the cell surface. However, less is known about the Duoxl\n                    <jats:italic>\/<\/jats:italic>\n                    Duoxal homologues. We identified four alternatively spliced Duoxal variants and explored their roles in Duox subcellular targeting and reconstitution. Duoxl and Duox2 are functionally rescued by Duoxa2 or the Duoxal variants that contain the third coding exon. All active maturation factors are cotransported to the cell surface when coexpressed with either Duoxl or Duox2, consistent with detection of endogenous Duoxal on apical plasma membranes of the airway epithelium. In contrast, the Duoxa proteins are retained in the endoplasmic reticulum when expressed without Duox. Duoxl\/Duoxala and Duox2\/Duoxa2 pairs produce the highest levels of hydrogen peroxide, as they undergo Golgi\u2010based carbohydrate modifications and form stable cell surface complexes. Cross\u2010functioning pairs that do not form stable complexes produce less hydrogen peroxide and leak superoxide. These findings suggest Duox activators not only promote Duox maturation, but they function as part of the hydrogen peroxide\u2010generating enzyme.\u2014Morand, S., Ueyama, T., Tsujibe, S., Saito, N., Korzeniowska, A., Leto, T. L. Duox maturation factors form cell surface complexes with Duox affecting the specificity of reactive oxygen species generation.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 23, 1205\u20131218 (2009)\n                  <\/jats:p>","DOI":"10.1096\/fj.08-120006","type":"journal-article","created":{"date-parts":[[2008,12,12]],"date-time":"2008-12-12T21:35:41Z","timestamp":1229117741000},"page":"1205-1218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":158,"title":["Duox maturation factors form cell surface complexes with Duox affecting the specificity of reactive oxygen species generation"],"prefix":"10.1096","volume":"23","author":[{"given":"Stanislas","family":"Morand","sequence":"first","affiliation":[{"name":"Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases National Institutes of Health Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiko","family":"Ueyama","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology Biosignal Research Center Kobe University Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Tsujibe","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology Biosignal Research Center Kobe University Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pharmacology Biosignal Research Center Kobe University Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Korzeniowska","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases National Institutes of Health Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Leto","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases National Institutes of Health Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2005.1767"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.52.37265"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000916200"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012752"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.9372"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.058321"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-1104fje"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.10.014"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3671"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200103132"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.048"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2004.03.008"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0302OC"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500516200"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1016\/S0021-9258(19)67857-9","article-title":"Mechanism of hydrogen peroxide formation catalyzed by NADPH oxidase in thyroid plasma membrane","volume":"266","author":"Dupuy C.","year":"1991","journal-title":"J. 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