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Here we show that human neutrophils (PMNs) challenged with LTB\n                    <jats:sub>4<\/jats:sub>\n                    (30 s to 5 min) release HBP as determined by Western blot analysis. This response peaks at 100 nM of agonist and is mediated by the BLT1 receptor. Proteinphosphatase\u20101 (30 \u03bcM) and wortmannin (0.5 \u03bcM) block the LTB\n                    <jats:sub>4<\/jats:sub>\n                    \u2010mediated HBP release from PMNs, which suggests involvement of the 1\u2010phosphatidylinositol 3\u2010kinase intracellular pathway during degranulation. Furthermore, postsecretory supernatants from LTB\n                    <jats:sub>4<\/jats:sub>\n                    \u2010stimulated PMNs induce intracellular calcium mobilization in endothelial cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    and increase in vascular permeability\n                    <jats:italic>in vivo<\/jats:italic>\n                    , as assessed in a mouse model of pleurisy. Selective removal of HBP from the supernatant significantly reduces these activities attributing a key role to HBP in the LTB\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced change in vascular permeability. This lipid\u2010protein axis could offer novel opportunities for pharmacological intervention in key steps of the vascular response to inflammation.\u2014Di Gennaro, A., Kenne, E., Wan, M., Soehnlein, O., Lindbom, L., Haeggstrom, J.Z. Leukotriene B4\u2010induced changes in vascular permeability are mediated by neutrophil release of heparinbinding protein (HBP\/CAP37\/azurocidin). FASEB J. 23, 1750\u20131757 (2009)\n                  <\/jats:p>","DOI":"10.1096\/fj.08-121277","type":"journal-article","created":{"date-parts":[[2009,1,16]],"date-time":"2009-01-16T22:02:28Z","timestamp":1232143348000},"page":"1750-1757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Leukotriene B\n                    <sub>4<\/sub>\n                    \u2010 induced changes in vascular permeability are mediated by neutrophil release of heparin\u2010binding protein (HBP\/CAP37\/azurocidin)"],"prefix":"10.1096","volume":"23","author":[{"given":"Antonio","family":"Di Gennaro","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics Division of Chemistry 2 Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellinor","family":"Kenne","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics Division of Chemistry 2 Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Soehnlein","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Lindbom","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper Z.","family":"Haeggstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics Division of Chemistry 2 Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6301011"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2820055"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02737601"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1980.tb12985.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5548.1871"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(84)90117-4"},{"key":"e_1_2_6_8_1","first-page":"53","article-title":"Leukotriene B4 is a complete secretagogue in human neutrophils: Ca2+ translocation in liposomes and kinetics of neutrophil activation","volume":"11","author":"Serhan C.N.","year":"1983","journal-title":"Adv. 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