{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:31:49Z","timestamp":1789201909032,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,5,26]],"date-time":"2009-05-26T00:00:00Z","timestamp":1243296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Drosophila<\/jats:italic>\n                    pericentrin\u2010like protein has been shown to be essential for the formation of the sensory cilia of chemosensory and mechanosensory neurons by mutant analysis in flies, while the\n                    <jats:italic>in vivo<\/jats:italic>\n                    function of pericentrin, a well\u2010studied mammalian centrosomal protein related to microcephalic primordial dwarfism, has been unclear. To determine whether pericentrin is required for ciliogenesis in mammals, we generated and analyzed mice with a hypomorphic mutation of\n                    <jats:italic>Pent<\/jats:italic>\n                    encoding the mouse pericentrin. Immunofluorescence analysis demonstrated that olfactory cilia of chemosensory neurons in the nasal olfactory epithelium were malformed in the homozygous mutant mice. On the other hand, the assembly of motile and primary cilia of non\u2010neuronal epithelial cells and the formation of sperm flagella were not affected in the Pent\u2010mutant mice. The defective assembly of olfactory cilia in the mutant was apparent from birth. The mutant animals displayed reduced olfactory performance in agreement with the compromised assembly of olfactory cilia. Our findings suggest that pericentrin is essential for the assembly of chemosensory cilia of olfactory receptor neurons, but it is not globally required for cilia formation in mammals.\u2014Miyoshi, K., Kasahara, K., Miyazaki, I., Shimizu, S., Taniguchi, M., Matsuzaki, S., Tohyama, M., Asanuma, M. Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 23, 3289\u20133297 (2009).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.08-124420","type":"journal-article","created":{"date-parts":[[2009,5,26]],"date-time":"2009-05-26T22:13:28Z","timestamp":1243376008000},"page":"3289-3297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice"],"prefix":"10.1096","volume":"23","author":[{"given":"Ko","family":"Miyoshi","sequence":"first","affiliation":[{"name":"Department of Brain Science Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyosuke","family":"Kasahara","sequence":"additional","affiliation":[{"name":"Department of Brain Science Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuko","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Brain Science Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoko","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience and Graduate School of Medicine Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience and Graduate School of Medicine Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinsuke","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience and Graduate School of Medicine Osaka University Osaka Japan"},{"name":"The Osaka-Hamamatsu Joint Research Center for Child Mental Development Graduate School of Medicine Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Tohyama","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience and Graduate School of Medicine Osaka University Osaka Japan"},{"name":"The Osaka-Hamamatsu Joint Research Center for Child Mental Development Graduate School of Medicine Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Asanuma","sequence":"additional","affiliation":[{"name":"Department of Brain Science Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,5,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90504-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.24.17267"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.5919"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1242\/jcs.114.4.797","article-title":"Kendrin\/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1","volume":"114","author":"Li Q.","year":"2001","journal-title":"J. Cell Sci."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvd105"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.1.163"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1091\/mbc.e02-02-0112","article-title":"Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring \u03b3-tubulin ring complex","volume":"13","author":"Takahashi M.","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1091\/mbc.e03-11-0796","article-title":"Mitosis-specific anchoring of \u03b3 tubulin complexes by pericentrin controls spindle organization and mitotic entry","volume":"15","author":"Zimmerman W. C.","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200402130"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1091\/mbc.e03-03-0191","article-title":"Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP","volume":"15","author":"Kawaguchi S.","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2255909"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80200-7"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80435-3"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00060-X"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-006-0065-8"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200405023"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2007.80"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151174"},{"key":"e_1_2_5_20_1","first-page":"737","article-title":"Exchangeable gene trap using the Cre\/mutated lox system","volume":"45","author":"Araki K.","year":"1999","journal-title":"Cell. Mol. Biol. (Noisy-le-grand)."},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2005.00792.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00611.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0888-7543(03)00119-8"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.10.101"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm952"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700253"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.12.151"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8191288"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.3.709"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.3.589"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01189"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700207"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1418"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405496101"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1644"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402354101"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.053"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00282"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn277"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200710085"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.08-124420","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.08-124420","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.08-124420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:45:46Z","timestamp":1761673546000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.08-124420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5,26]]},"references-count":39,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1096\/fj.08-124420"],"URL":"https:\/\/doi.org\/10.1096\/fj.08-124420","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5,26]]},"assertion":[{"value":"2008-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}