{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:32:35Z","timestamp":1790861555878,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T00:00:00Z","timestamp":1246233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001030","name":"National Heart Foundation of Australia","doi-asserted-by":"publisher","award":["G 08M 3695"],"award-info":[{"award-number":["G 08M 3695"]}],"id":[{"id":"10.13039\/501100001030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Activation of the heterotrimeric G protein Gq causes cardiomyocyte hypertrophy\n                    <jats:italic>in vivo<\/jats:italic>\n                    and in cell culture models. Hypertrophic responses induced by pressure or volume overload are exacerbated by increased Gq activity and ameliorated by Gq inhibition. Gq activates phospholipase C\u03b2 (PLC\u03b2) subtypes, resulting in generation of the intracellular messengers inositol(1,4,5)\n                    <jats:italic>tris<\/jats:italic>\n                    \u2010phosphate [Ins(1,4,5)P\n                    <jats:sub>3<\/jats:sub>\n                    ] and sn\u20101,2\u2010diacylglycerol (DAG), which regulate intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and conventional protein kinase C subtypes, respectively. Gq can also signal independently of PLC\u03b2, and the involvement of either Ins(1,4,5)P\n                    <jats:sub>3<\/jats:sub>\n                    or DAG in cardiomyocyte hypertrophy has not been unequivocally established. Overexpression of one splice variant of PLC\u03b21, specifically PLC\u03b21b, in neonatal rat cardiomyocytes causes increased cell size, elevated protein\/ DNA ratio, and heightened expression of the hypertrophy\u2010related marker gene, atrial natriuretic peptide. The other splice variant, PLC\u03b21a, had no effect. Expression of a 32\u2010aa C\u2010terminal PLC\u03b21b peptide, which competes with PLC\u03b21b for sarcolemmal association, prevented PLC activation and eliminated hypertrophic responses initiated by Gq or Gq\u2010coupled \u03b1\n                    <jats:sub>1<\/jats:sub>\n                    \u2010adrenergic receptors. In contrast, a PLC\u03b21a C\u2010terminal peptide altered neither PLC activity nor cellular hypertrophy. We conclude that hypertrophic responses initiated by Gq are mediated specifically by PLC\u03b21b. Preventing PLC\u03b21b association with the sarcolemma may provide a useful therapeutic target to limit hypertrophy.\u2014Filtz, T. M., Grubb, D. R., McLeod\u2010Dryden, T. J., Luo, J., Woodcock, E. A. Gq\u2010initiated cardiomyocyte hypertrophy is mediated by phospholipase C\u03b21b.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 23, 3564\u20133570 (2009).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-133983","type":"journal-article","created":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T20:46:34Z","timestamp":1246308394000},"page":"3564-3570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Gq\u2010initiated cardiomyocyte hypertrophy is mediated by phospholipase C\u03b21b"],"prefix":"10.1096","volume":"23","author":[{"given":"Theresa M.","family":"Filtz","sequence":"first","affiliation":[{"name":"Molecular Cardiology Laboratory Baker IDI Heart and Diabetes Institute Melbourne Victoria Australia"},{"name":"Department of Pharmaceutical Sciences Oregon State University 203 Pharmacy Bldg. Corvallis OR 97331 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Grubb","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Laboratory Baker IDI Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany J.","family":"McLeod-Dryden","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Laboratory Baker IDI Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieting","family":"Luo","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Laboratory Baker IDI Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Woodcock","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Laboratory Baker IDI Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,29]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"3824","article-title":"Transition from hypertrophy to failure","volume":"96","author":"Lorell B.","year":"1997","journal-title":"Circulation"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.56.6.884"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/S0021-9258(17)42228-9","article-title":"Endothelin-1 and fibroblast growth factors stimulate the mitogen-activated protein kinase signaling cascade in cardiac myocytes: the potential role of the cascade in the integration of 2 signaling pathways leading to myocyte hypertrophy","volume":"269","author":"Bogoyevitch M. A.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.3.413"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.931"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(99)00004-3"},{"key":"e_1_2_5_8_1","first-page":"65","article-title":"Genetic alterations that inhibit in vivo pressure-overload hypertrophy prevent cardiac dysfunction despite increased wall stress","volume":"50","author":"Esposito G.","year":"2001","journal-title":"Cardiovasc. Res."},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1101-1236"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1016\/S0021-9258(18)46018-8","article-title":"Activation of phospholipaseC by the a subunits of the Gq and G11 proteins in transfected cos-7 cells","volume":"25","author":"Wu D.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.281"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(94)90186-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402320200"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27374"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00066.2008"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204715200"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411322200"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703458200"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1997.0613"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.49.31185"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106572200"},{"key":"e_1_2_5_22_1","doi-asserted-by":"crossref","first-page":"8240","DOI":"10.1016\/S0021-9258(17)37185-5","article-title":"Two forms of phospholipase C-\u03b21 generated by alternative splicing","volume":"269","author":"Bahk Y. Y.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-102558"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.24.8318-8328.2001"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006871722652"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2002"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_5_29_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0022-2828(03)00009-9","article-title":"UTP but not ATP causes hypertrophic growth in neonatal cardiomyocytes","volume":"35","author":"Pham T.","year":"2003","journal-title":"J. Mol. Cell. Cardiol."},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000196578.15385.bb"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204275"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.51.6.787"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506810200"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.03.011"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.4.458"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh0202.104109"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310012200"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414212200"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.391"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605536200"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27702"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142505"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601417"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712265105"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.19.8374-8385.2004"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.72.309"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.12.007"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004630200"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.9.2981-2990.2001"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5363.574"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-133983","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.09-133983","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-133983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:45:24Z","timestamp":1761673524000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.09-133983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,29]]},"references-count":49,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1096\/fj.09-133983"],"URL":"https:\/\/doi.org\/10.1096\/fj.09-133983","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,29]]},"assertion":[{"value":"2009-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}