{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:47:46Z","timestamp":1782744466464,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T00:00:00Z","timestamp":1256515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T00:00:00Z","timestamp":1256515200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI050775"],"award-info":[{"award-number":["AI050775"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>\n                    Genetic background significantly affects angiogenesis in mice. However, lymphangiogenic response to growth factors (GFs) in different strains has not been studied. We report constitutive expression of corneal lymphatics that extends beyond the limits of normal limbal vessels. In untreated corneas, the total number (P=0.006), the number above blood vessels (P=10\n                    <jats:sup>\u22128<\/jats:sup>\n                    ), and the area of preexisting lymphatics (P= 0.007) were significantly higher in C57BL\/6 than in BALB\/c mice. Normal corneas of three other strains, the\n                    <jats:italic>nu\/nu<\/jats:italic>\n                    , 129E, and Black Swiss mice, showed in most parameters intermediate phenotypes.\n                    <jats:italic>FGF\u20102<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice showed significantly less preexisting lymphatics than control (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.009), which suggests a role for this GF in lymphatic development. VEGF\u2010A\u2010induced corneal lymphangiogenic response was significantly higher in BALB\/c mice (P=0.03), but it did not differ significantly in C57BL\/6 mice, when compared to PBS\u2010implanted control. FGFR\u20103 expression was higher in C57BL\/6 than BALB\/c mice, which suggests GF\u2010receptor heterogeneity as a possible explanation for strain\u2010dependent differences. The heterogeneity of preexisting lymphatic vessels in the limbal area significantly correlated with the extent of corneal lymphangiogenesis (VEGF\u2010A\n                    <jats:italic>r<\/jats:italic>\n                    =0.7,\n                    <jats:italic>P<\/jats:italic>\n                    =0.01;FGF\u20102:\n                    <jats:italic>r<\/jats:italic>\n                    =0.96,\n                    <jats:italic>P<\/jats:italic>\n                    =10\n                    <jats:sup>\u22125<\/jats:sup>\n                    ) in BALB\/c but not in C57BL\/6 mice. Removal of conjunctival lymphatics did not affect GF\u2010induced lymphangiogenesis. This work introduces physiological expression of lymphatics without blood vessels, which indicates that angiogenesis and lymphangiogenesis, even though intricately related, may occur independently. Furthermore, we show strain\u2010dependence of normal and GF\u2010induced lymphangiogenesis. These differences may affect disease development in various strains.\u2014Nakao, S., Maruyama, K., Zandi, S., Melhorn, M. I., Taher, M., Noda, K., Nusayr, E., Doetschman, T., Hafezi\u2010Moghadam, A. Lymphangiogenesis and angiogenesis: concurrence and\/or dependence? Studies in inbred mouse strains.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 504\u2013513 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-134056","type":"journal-article","created":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T20:56:43Z","timestamp":1256590603000},"page":"504-513","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Lymphangiogenesis and angiogenesis: concurrence and\/or dependence? Studies in inbred mouse strains"],"prefix":"10.1096","volume":"24","author":[{"given":"Shintaro","family":"Nakao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuichi","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kyoto Prefectural University of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souska","family":"Zandi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark I.","family":"Melhorn","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Taher","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Noda","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eyad","family":"Nusayr","sequence":"additional","affiliation":[{"name":"BIO5 Institute and Department of Cell Biology and Anatomy University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Doetschman","sequence":"additional","affiliation":[{"name":"BIO5 Institute and Department of Cell Biology and Anatomy University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafezi\u2010Moghadam","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Harvard Medical School and Massachusetts Eye and Ear Infirmary Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,10,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05249"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000214230.21238.3d"},{"key":"e_1_2_5_4_1","first-page":"142","article-title":"An experimental study of the new growth of lymphatic vessels in the cornea","volume":"24","author":"Aoki K.","year":"1951","journal-title":"Kaibogaku Zasshi"},{"key":"e_1_2_5_5_1","first-page":"337","article-title":"Endothelial cell lined lymphatics in the vascularized rabbit cornea","volume":"5","author":"Collin H. 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