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Pgp may be a candidate target for new anthelmintics, as it plays critical roles in normal cell physiology, in removal of drugs from cells, and potentially in the development of drug resistance. Schistosomes are parasitic flatworms that cause schistosomiasis, which affects hundreds of millions of people worldwide. Here, we express SMDR2, a Pgp homologue from\n                    <jats:italic>Schistosoma mansoni<\/jats:italic>\n                    (Platyhelminthes), in Chinese hamster ovary (CHO) cells and use fluorescence\u2010based assays to examine the functional and pharmacological properties of this transporter. Membrane vesicles from stably transfected CHO cells expressing recombinant SMDR2 show significant increases in rhodamine transport and ATP hydrolysis compared with those from control cells or cells transfected with empty vector. SMDR2\u2010mediated transport is inhibited by the Pgp modulators verapamil (IC\n                    <jats:sub>50<\/jats:sub>\n                    =12.1 \u03bcM) and nifedipine, and also by praziqu\u2010antel, the current drug of choice against schisotosomia\u2010sis (IC\n                    <jats:sub>50<\/jats:sub>\n                    =17.4 \u03bcM). Efflux measurements of a fluorescent analog of praziquantel indicate that it is also a substrate for SMDR2. The interaction of praziquantel with SMDR2 may offer new strategies for potentiating the action of praziquantel and possibly overcoming drug resistance.\u2014Kasinathan, R. S., Goronga, T., Messerli, S. M., Webb, T. R., Greenberg, R. M. Modulation of a Schistosoma mansoni multidrug transporter by the antischistosomal drug praziquantel.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 128\u2013135 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-137091","type":"journal-article","created":{"date-parts":[[2009,9,2]],"date-time":"2009-09-02T22:10:39Z","timestamp":1251929439000},"page":"128-135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Modulation of a\n                    <i>Schistosoma mansoni<\/i>\n                    multidrug transporter by the antischistosomal drug praziquantel"],"prefix":"10.1096","volume":"24","author":[{"given":"Ravi S.","family":"Kasinathan","sequence":"first","affiliation":[{"name":"Department of Pathobiology School of Veterinary Medicine University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinopiwa","family":"Goronga","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Therapeutics St. Jude Children's Research Hospital Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanta M.","family":"Messerli","sequence":"additional","affiliation":[{"name":"Marine Biological Laboratory Woods Hole Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Therapeutics St. Jude Children's Research Hospital Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Department of Pathobiology School of Veterinary Medicine University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00045-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro801"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69440-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2009.05.003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(85)90020-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.09.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1017\/S0031182005008346","article-title":"Ca2+ signalling, voltage-gated Ca2+ channels and praziquantel in flatworm neuromusculature","volume":"131","author":"Greenberg R. 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