{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:56:41Z","timestamp":1790791001366,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T00:00:00Z","timestamp":1253664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dengue fever is an emerging viral disease transmitted by arthropods to humans in tropical countries. Dengue hemorrhagic fever (DHF) is escalating in frequency and mortality rates. Here we studied the involvement of macrophage migration inhibitory factor (MIF) in dengue virus (DENV) infection and its pathogenesis. Patients with DHF had elevated plasma concentrations of MIF. Both leukocytes from these patients and macrophages from healthy donors infected\n                    <jats:italic>in vitro<\/jats:italic>\n                    with DENV showed a substantial amount of MIF within lipid droplets. The secretion of MIF by macrophages and hepatocytes required a productive infection and occurred without an increase in gene transcription or cell death, thus indicating active secretion from preformed stocks.\n                    <jats:italic>In vivo<\/jats:italic>\n                    infection of wild\u2010type and\n                    <jats:italic>miF<\/jats:italic>\n                    deficient\n                    <jats:italic>\n                      (Mif\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      )\n                    <\/jats:italic>\n                    mice demonstrated a role of MIF in dengue pathogenesis. Clinical disease was less severe in\n                    <jats:italic>\n                      Mif\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice, and they exhibited a significant delay in lethality, lower viremia, and lower viral load in the spleen than wild\u2010type mice. This reduction in all parameters of severity on DENV infection in\n                    <jats:italic>\n                      Mif\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice correlated with reduced proinflam\u2010matory cytokine concentrations. These results demon\u2010strated the contribution of MIF to the pathogenesis of dengue and pointed to a possible beneficial role of neutralizing MIF as an adjunctive therapeutic approach to treat the severe forms of the disease.\u2014Assunc\u00e4o\u2010Miranda, I., Amaral, F. A., Bozza, F. A., Fagundes, C. T., Sousa, L. P., Souza, D. G., Pacheco, P., Barbosa\u2010Lima, G., Gomes, R. N., Bozza, P. T., Da Poian, A. T., Teixeira, M. M., Bozza, M. T. Contribution of macro\u2010phage migration inhibitory factor to the pathogenesis of dengue virus infection.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 218\u2013228 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-139469","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T22:20:07Z","timestamp":1253744407000},"page":"218-228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":105,"title":["Contribution of macrophage migration inhibitory factor to the pathogenesis of dengue virus infection"],"prefix":"10.1096","volume":"24","author":[{"given":"Iranaia","family":"Assun\u00e7\u00e3o-Miranda","sequence":"first","affiliation":[{"name":"Programa de Biolog\u00eda Estrutural Instituto de Bioqu\u00edmica M\u00e9dica Rio de Janeiro Brazil"},{"name":"Departamento de Imunologia Instituto de Microbiologia Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio A.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando A.","family":"Bozza","sequence":"additional","affiliation":[{"name":"Intensive Care Unit Instituto de Pesquisa Clinica Evandro Chagas Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio T.","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirlandia P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle G.","family":"Souza","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia Instituto Oswaldo Cruz Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giselle","family":"Barbosa-Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia Instituto Oswaldo Cruz Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel N.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia Instituto Oswaldo Cruz Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia T.","family":"Bozza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia Instituto Oswaldo Cruz Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea T. Da","family":"Poian","sequence":"additional","affiliation":[{"name":"Programa de Biolog\u00eda Estrutural Instituto de Bioqu\u00edmica M\u00e9dica Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro M.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo T.","family":"Bozza","sequence":"additional","affiliation":[{"name":"Departamento de Imunologia Instituto de Microbiologia Universidade Federal do Rio de Janeiro (UFRJ) Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.324.7353.1563"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00171-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1144"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.03.001"},{"key":"e_1_2_6_6_1","unstructured":"World Health Organization. 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