{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:03:09Z","timestamp":1774123389989,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T00:00:00Z","timestamp":1255478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004330","name":"GlaxoSmithKline","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004330","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005633","name":"King Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The primary aim of this study was to investigate adenosine receptors (ARs) in bronchoalveolar lavage (BAL) macrophages from patients with chronic obstructive pulmonary disease (COPD) and age\u2010matched healthy smokers. A\n                    <jats:sub>2B<\/jats:sub>\n                    ARs were significantly decreased in BAL macrophages from patients with COPD when compared with healthy smokers. The effect of proinflammatory cytokines and oxidative\/ nitrosative stress on AR expression and function in U937 cells before and after PMA treatment was evaluated. IL\u20101 \u03b2 and TNF\u2010\u03b1 treatment up\u2010regulated A\n                    <jats:sub>2A<\/jats:sub>\n                    \u2010 and A\n                    <jats:sub>3<\/jats:sub>\n                    ARs but not A\n                    <jats:sub>1<\/jats:sub>\n                    \u2010 or A\n                    <jats:sub>2B<\/jats:sub>\n                    ARs, whereas IL\u20106 did not modify AR expression. In contrast, oxidative\/nitrosative stress selectively decreased A\n                    <jats:sub>2B<\/jats:sub>\n                    AR expression, which was associated with a reduction in the potency of the adenosine agonist 5\u2032\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010ethylcarboxamideadenosine (NECA) to induce cAMP. Further, the ability of NECA to enhance cell proliferation was increased after oxidative\/nitrosative stress. The specific involvement of A\n                    <jats:sub>2B<\/jats:sub>\n                    ARs was investigated by using potent and selective A\n                    <jats:sub>2B<\/jats:sub>\n                    AR antagonist and by A\n                    <jats:sub>2B<\/jats:sub>\n                    AR knockdown using siRNA and demonstrated responses similar to those obtained with oxidative\/nitrosative stress.\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylcysteine (NAC), an antioxidant agent, counteracted the decrease in A\n                    <jats:sub>2B<\/jats:sub>\n                    AR expression, as well as the altered NECA effects on cAMP and cell proliferation. These findings highlight the central role of A\n                    <jats:sub>2B<\/jats:sub>\n                    ARs in alveolar macrophages, suggesting that their modulation could represent an innovative pharmacological strategy to manage COPD.\u2014Varani, K., Caramori, G., Vincenzi, F., Tosi, A., Barczyk, A., Contoli, M., Casolari, P., Triggiani, M., Hansel, T., Leung, E., MacLennan, S., Barnes, P. J., Fan Chung, K., Adcock, I., Papi, A., Borea, P. A. Oxidative\/nitrosative stress selectively altered A\n                    <jats:sub>2B<\/jats:sub>\n                    adenosine receptors in chronic obstructive pulmonary disease.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    24, 1192\u20131204 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-139485","type":"journal-article","created":{"date-parts":[[2009,12,14]],"date-time":"2009-12-14T21:48:55Z","timestamp":1260827335000},"page":"1192-1204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Oxidative\/nitrosative stress selectively altered A\n                    <sub>2B<\/sub>\n                    adenosine receptors in chronic obstructive pulmonary disease"],"prefix":"10.1096","volume":"24","author":[{"given":"Katia","family":"Varani","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Medicine Pharmacology Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaetano","family":"Caramori","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Respiratory Disease Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Vincenzi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Pharmacology Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Tosi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Pharmacology Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Barczyk","sequence":"additional","affiliation":[{"name":"Katedra i Klinika Pneumonologii Slaskiej Akademii Medycznej Katowice Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Contoli","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Respiratory Disease Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Casolari","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Respiratory Disease Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Triggiani","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology and Allergy University of Naples Federico II Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor","family":"Hansel","sequence":"additional","affiliation":[{"name":"Airways Disease Section National Heart and Lung Institute Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Leung","sequence":"additional","affiliation":[{"name":"King Pharmaceuticals Cary North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"MacLennan","sequence":"additional","affiliation":[{"name":"King Pharmaceuticals Cary North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Airways Disease Section National Heart and Lung Institute Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kian Fan","family":"Chung","sequence":"additional","affiliation":[{"name":"Airways Disease Section National Heart and Lung Institute Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Adcock","sequence":"additional","affiliation":[{"name":"Airways Disease Section National Heart and Lung Institute Imperial College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Papi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Respiratory Disease Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pier Andrea","family":"Borea","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Pharmacology Section University of Ferrara Ferrara Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,10,14]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1","volume-title":"NHLBI\/WHO Workshop Report, NIH Publication","author":"Global Initiative for Chronic Obstructive Lung Disease.","year":"2001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2009.04.003"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/187153008786848303"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200506-869OC"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612054065774"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.06.034"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200411-056SF"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1602410"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.02.004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032158"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"675","DOI":"10.2174\/156801005774912833","article-title":"Emerging targets for COPD therapy","volume":"4","author":"Barnes P. J.","year":"2005","journal-title":"Curr. Drug Targets Inflamm. Allergy."},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1111\/j.1365-2222.2004.02030.x","article-title":"Cellular and molecular mechanisms in chronic obstructive pulmonary disease: an overview","volume":"34","author":"Stefano A.","year":"2004","journal-title":"Clin. Exp. Allergy"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200502000-00014"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.5.9910112"},{"key":"e_1_2_6_16_1","first-page":"5","article-title":"Lung volumes and forced ventilatory flows. Report Working Party, standardization of lung function tests, European Community for Steel and Coal, official statement of the European Respiratory Society","volume":"16","author":"Quanjer P.","year":"1993","journal-title":"Eur. Respir. J."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20040416"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103604200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.18.6891-6903.2000"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(88)90050-1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/S0026-895X(24)26507-X","article-title":"[3H]-MRE 3008-F20: a novel antagonist radioligand for the pharmacological and biochemical characterization of human A3 adenosine receptors","volume":"57","author":"Varani K.","year":"2000","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0543fje"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1124\/mol.104.009225","article-title":"Expression, pharmacological profile and functional coupling of A2B receptors in a recombinant system and in peripheral blood cells by using a novel selective antagonist radioligand, [3H]-MRE 2029-F20","volume":"67","author":"Gessi S.","year":"2005","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.08.018"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413772200"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1056\/NEJMoa041892","article-title":"Decreased histone deacetylase activity in chronic obstructive pulmonary disease","volume":"12","author":"Ito K.","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024157508492"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90515-1"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200004)190:5<619::AID-PATH555>3.0.CO;2-6"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/15412550902724164"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.2104010"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.7.4026"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.10.7173"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0255OC"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23094"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.046"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(02)00052-4"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00206.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.12.059"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa063070"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67100-0"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27303"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.227"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900515"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-139485","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.09-139485","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-139485","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T21:31:18Z","timestamp":1761687078000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.09-139485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,14]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1096\/fj.09-139485"],"URL":"https:\/\/doi.org\/10.1096\/fj.09-139485","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10,14]]},"assertion":[{"value":"2009-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}