{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T15:58:58Z","timestamp":1794412738569,"version":"4.9.0"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,3,19]],"date-time":"2010-03-19T00:00:00Z","timestamp":1268956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL67033"],"award-info":[{"award-number":["HL67033"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL82774"],"award-info":[{"award-number":["HL82774"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL77288"],"award-info":[{"award-number":["HL77288"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL094451"],"award-info":[{"award-number":["HL094451"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL74966"],"award-info":[{"award-number":["HL74966"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hyperhomocysteinemia is an independent risk factor for cardiovascular disease. Homocysteine (Hcy) metabolism involves multiple enzymes; however, tissue Hcy metabolism and its relevance to methylation remain unknown. Here, we established gene expression profiles of 8 Hcy metabolic and 12 methylation enzymes in 20 human and 19 mouse tissues through bioinformatic analysis using expression sequence tag clone counts in tissue cDNA libraries. We analyzed correlations between gene expression, Hcy,\n                    <jats:italic>S<\/jats:italic>\n                    \u2010adenosylhomocysteine (SAH), and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010adenosylmethionine (SAM) levels, and SAM\/SAH ratios in mouse tissues. Hcy metabolic and methylation enzymes were classified into two types. The expression of Type 1 enzymes positively correlated with tissue Hcy and SAH levels. These include cystathionine \u03b2\u2010synthase, cystathionine\u2010\u03b3\u2010lyase, paraxonase 1, 5,10\u2010methylenetetrahydrofolate reductase, betaine:homocysteine methyltransferase, methionine adenosyl\u2010transferase, phosphatidylethanolamine\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyltransferases and glycine\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyltransferase. Type 2 enzyme expressions correlate with neither tissue Hcy nor SAH levels. These include SAH hydrolase, methionyl\u2010tRNA synthase, 5\u2010methyltetrahydrofolate:Hcy methyltransferase,\n                    <jats:italic>S<\/jats:italic>\n                    \u2010adenosylmethionine decarboxylase, DNA methyltransferase 1\/3a, isoprenylcysteine carboxyl methyltransferases, and histone\u2010lysine\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyltransferase. SAH is the only Hcy metabolite significantly correlated with Hcy levels and methylation enzyme expression. We established equations expressing combined effects of methylation enzymes on tissue SAH, SAM, and SAM\/SAH ratios. Our study is the first to provide panoramic tissue gene expression profiles and mathematical models of tissue methylation regulation.\u2014Chen, N. C., Yang, F., Capecci, L. M., Gu, Z., Schafer, A. I., Durante, W., Yang, X.\u2010F., Wang, H. Regulation of homocysteine metabolism and methylation in human and mouse tissues.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 2804\u20132817 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-143651","type":"journal-article","created":{"date-parts":[[2010,3,19]],"date-time":"2010-03-19T22:18:20Z","timestamp":1269037100000},"page":"2804-2817","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":132,"title":["Regulation of homocysteine metabolism and methylation in human and mouse tissues"],"prefix":"10.1096","volume":"24","author":[{"given":"Natalie C.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis M.","family":"Capecci","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyu","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew I.","family":"Schafer","sequence":"additional","affiliation":[{"name":"Department of Medicine Weill Cornell Medical College  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Durante","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Feng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"},{"name":"Cardiovascular Research Center Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Temple University School of Medicine  Philadelphia Pennsylvania USA"},{"name":"Cardiovascular Research Center Temple University School of Medicine  Philadelphia Pennsylvania USA"},{"name":"Thrombosis Research Center Temple University School of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi224"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.GIM.0000095001.12570.A8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.5.433"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.40.25380"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-06-096701"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711450769196X"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32011"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000258855.60637.58"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2009.102"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.3.939.h80302000939_939_945"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(99)00002-X"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.350"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116485"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.31"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1681\/ASN.V8121899","article-title":"Metabolic consequences of folate\u2010induced reduction of hyperhomocysteinemia in uremia","volume":"8","author":"Perna A. F.","year":"1997","journal-title":"J. Am. Soc. Nephrol."},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1016\/S0021-9258(17)43360-6","article-title":"Homocysteine in tissues of the mouse and rat","volume":"259","author":"Ueland P. M.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1016\/S0021-9258(17)35762-9","article-title":"Determination of in vivo protein binding of homocysteine and its relation to free homocysteine in the liver and other tissues of the rat","volume":"261","author":"Svardal A.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_19_1","first-page":"4142","article-title":"Effect of 2\u2032\u2010deoxycoformycin infusion on S\u2010adenosylhomocysteine hydrolase and the amount of S\u2010adenosylhomocysteine and related compounds in tissues of mice","volume":"43","author":"Helland S.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_6_20_1","first-page":"1","volume-title":"The NCBI Handbook","author":"Pontius J.","year":"2003"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463200902200208"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2008.07.041"},{"key":"e_1_2_6_23_1","first-page":"1847","article-title":"S\u2010adenosylhomocysteine and S\u2010adenosylhomocysteine hydrolase in various tissues of mice given injection of 9\u2010beta\u2010D\u2010arabinofuranosyladenine","volume":"43","author":"Helland S.","year":"1983","journal-title":"Cancer Res."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.8.2157"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.11.2811"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.8.2361S"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.324"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.60.041807.123308"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.8.1833"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1214371"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0741-8329(96)00030-4"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3500685"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"S67","DOI":"10.1007\/PL00014309","article-title":"Clinical aspects of cystathionine beta\u2010synthase deficiency: how wide is the spectrum? The Italian Collaborative Study Group on Homocystinuria","volume":"2","author":"De Franchis R.","year":"1998","journal-title":"Eur. J. Pediatr. Suppl."},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0810551"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1585"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.6.1493"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/28406"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002725200"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-006-0008-1"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.275.1.G14"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.08.026"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(77)90070-3"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9810609"},{"key":"e_1_2_6_45_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/BF02534900","article-title":"Microsomal phosphatidylethanolamine methyltransferase: Inhibition by S\u2010adenosylhomocysteine","volume":"16","author":"Hoffman D. R.","year":"1980","journal-title":"Lipids"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)62211-2"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45067-9"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-09-3078"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408664200"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.1.210"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107553200"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.10.2968"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.24.8.1403"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2009.05.011"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e3181aaefd6"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-0087"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-143651","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.09-143651","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.09-143651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:32:16Z","timestamp":1761669136000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.09-143651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,19]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1096\/fj.09-143651"],"URL":"https:\/\/doi.org\/10.1096\/fj.09-143651","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,19]]},"assertion":[{"value":"2009-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}