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Common genetic variations alter the risk of developing organ dysfunction when choline deficient, probably by causing metabolic inefficiencies that should be detectable even while ingesting a normal choline\u2010adequate diet. We determined whether metabolomic profiling of plasma at baseline could predict whether humans will develop liver dysfunction when deprived of dietary choline. Fifty\u2010three participants were fed a diet containing 550 mg choline\/70 kg\/d for 10 d and then fed &lt;50 mg choline\/70 kg\/d for up to 42 d. Participants who developed organ dysfunction on this diet were repleted with a choline\u2010adequate diet for \u22653 d. Plasma samples, obtained at baseline, end of depletion, and end of repletion, were used for targeted and nontargeted metabolomic profiling. Liver fat was assessed using magnetic resonance spectroscopy. Metabolomic profiling and targeted biochemical analyses were highly correlated for the analytes assessed by both procedures. In addition, we report relative concentration changes of other small molecules detected by the nontargeted metabolomic analysis after choline depletion. Finally, we show that metabolomic profiles of participants when they were consuming a control baseline diet could predict whether they would develop liver dysfunction when deprived of dietary choline.\u2014Sha, W., da Costa, K., Fischer, L. M., Milburn, M. V., Lawton, K. A., Berger, A., Jia, W., Zeisel, S. H. Metabolomic profiling can predict which humans will develop liver dysfunction when deprived of dietary choline.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 2962\u20132975 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.09-154054","type":"journal-article","created":{"date-parts":[[2010,4,7]],"date-time":"2010-04-07T01:40:30Z","timestamp":1270604430000},"page":"2962-2975","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":109,"title":["Metabolomic profiling can predict which humans will develop liver dysfunction when deprived of dietary choline"],"prefix":"10.1096","volume":"24","author":[{"given":"Wei","family":"Sha","sequence":"first","affiliation":[{"name":"Bioinformatics Research Center University of North Carolina at Charlotte North Carolina Research Campus  Kannapolis North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerry\u2010Ann Da","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Nutrition School of Public Health and School of Medicine University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie M.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Nutrition School of Public Health and School of Medicine University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael V.","family":"Milburn","sequence":"additional","affiliation":[{"name":"Metabolon, Inc.  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