{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:36:48Z","timestamp":1791175008357,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,4,21]],"date-time":"2010-04-21T00:00:00Z","timestamp":1271808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM060000"],"award-info":[{"award-number":["GM060000"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006952","name":"Louisiana Board of Regents","doi-asserted-by":"publisher","award":["RC\/EEP\u201005(2007\u201010)"],"award-info":[{"award-number":["RC\/EEP\u201005(2007\u201010)"]}],"id":[{"id":"10.13039\/100006952","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Membrane\u2010permeabilizing peptide antibiotics are an underutilized weapon in the battle against drug\u2010resistant microorganisms. This is true, in part, because of the bottleneck caused by the lack of explicit design principles and the paucity of simple high\u2010throughput methods for selection. In this work, we characterize the requirements for broad\u2010spectrum antimicrobial activity by membrane permeabilization and find that different microbial membranes have very different susceptibilities to permeabilization by individual antimicrobial peptides. Broad\u2010spectrum activity requires only that an AMP have at least a small amount of membrane\u2010permeabilizing activity against multiple classes of microbes, a feature that we show to be rare in a peptide library containing many members with species\u2010specific activity. We compare biological and vesicle\u2010based high\u2010throughput strategies for selecting such broad\u2010spectrum AMPs from combinatorial peptide libraries and demonstrate that a simple\n                    <jats:italic>in vitro<\/jats:italic>\n                    , lipid vesicle\u2010based high\u2010throughput screen is the most effective strategy for rapid discovery of novel, broad\u2010spectrum antimicrobial peptides.\u2014Rathinakumar, R., Wimley, W. C. High\u2010throughput discovery of broad\u2010spectrum peptide antibiotics.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 24, 3232\u20133238 (2010).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-157040","type":"journal-article","created":{"date-parts":[[2010,4,21]],"date-time":"2010-04-21T22:46:30Z","timestamp":1271889990000},"page":"3232-3238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["High\u2010throughput discovery of broad\u2010spectrum peptide antibiotics"],"prefix":"10.1096","volume":"24","author":[{"given":"Ramesh","family":"Rathinakumar","sequence":"first","affiliation":[{"name":"Department of Biochemistry Tulane University Health Sciences Center  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Wimley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Tulane University Health Sciences Center  New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1312.070629"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp0804651"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2007.12.013"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-440X(95)80038-7"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1267"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401567101"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.1.2"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2009.07.004"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.03.009"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.10260"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi900914g"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8093247"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8017863"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2009.02.004"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1206"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi700978h"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.133488"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.1-6.2003"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502013102"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5137"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.521"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1995.tb01055.x"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.10-157040","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.10-157040","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.10-157040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:28:54Z","timestamp":1761694134000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.10-157040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,21]]},"references-count":22,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1096\/fj.10-157040"],"URL":"https:\/\/doi.org\/10.1096\/fj.10-157040","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,21]]},"assertion":[{"value":"2010-02-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}