{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T12:35:48Z","timestamp":1793104548057,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T00:00:00Z","timestamp":1285718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T00:00:00Z","timestamp":1285718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["LSHM\u2010CT\u20102004\u2013005272"],"award-info":[{"award-number":["LSHM\u2010CT\u20102004\u2013005272"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:p>\n                    AMP\u2010activated protein kinase (AMPK) is an \u03b1\u03b2\u03b3 heterotrimer conserved throughout evolution and important for energy sensing in all eukaryote cells. AMPK controls metabolism and various cellular events in response to both hormones and changes in cellular energy status. The \u03b3 subunit senses intracellular energy status through the competitive binding of AMP and ATP. We show here that targeted disruption of the mouse AMPKyl gene (\n                    <jats:italic>Prk\u03b1g1<\/jats:italic>\n                    ) causes regenerative hemolytic anemia by increasing the sequestration of abnormal erythrocytes.\n                    <jats:italic>\n                      Prk\u03b1g1\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    mice displayed splenomegaly and iron accumulation due to compensatory splenic erythropoiesis and erythrophagocytosis. Moreover\u2019 AMPKyl\u2010deficient eryth\u2010rocytes were highly resistant to osmotic hemolysis and poorly deformable in response to increasing shear stress\u2019 consistent with greater membrane rigidity. No change in cytoskeletal protein composition was observed;however\u2019 the phosphorylation level of adducin\u2019 a protein promoting the binding of spectrin to actin\u2019 was higher in AMPKyl\u2010deficient erythrocytes. Together\u2019 these results demonstrate that AMPKy1 subunit is required for the maintenance of erythrocyte membrane elasticity.\u2014Foretz, M., H\u00e9brard, S., Guihard, S., Leclerc, J., Do Cruzeiro, M., Hamard, G., Niedergang, F., Gaudry, M., Viollet, B. The AMPK\n                    <jats:italic>\u03b31<\/jats:italic>\n                    subunit plays an essential role in erythrocyte membrane elasticity\u2019 and its genetic inactivation induces splenomegaly and anemia.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    25, 337\u2013347 (2011).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-169383","type":"journal-article","created":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T23:47:28Z","timestamp":1285804048000},"page":"337-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["The AMPKyl subunit plays an essential role in erythrocyte membrane elasticity, and its genetic inactivation induces splenomegaly and anemia"],"prefix":"10.1096","volume":"25","author":[{"given":"Marc","family":"Foretz","sequence":"first","affiliation":[{"name":"Institut Cochin Universit\u00e9 Paris Descartes Centre National de la Recherche Scientifique (CNRS) UMR 8104 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1016 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"H\u00e9brard","sequence":"additional","affiliation":[{"name":"Institut Cochin Universit\u00e9 Paris Descartes Centre National de la Recherche Scientifique (CNRS) UMR 8104 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1016 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soizic","family":"Guihard","sequence":"additional","affiliation":[{"name":"Institut Cochin Universit\u00e9 Paris Descartes Centre National de la Recherche Scientifique (CNRS) UMR 8104 Paris 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Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1016 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Viollet","sequence":"additional","affiliation":[{"name":"Institut Cochin Universit\u00e9 Paris Descartes Centre National de la Recherche Scientifique (CNRS) UMR 8104 Paris France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1016 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41951-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.611"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-4924-2-28"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.05.009"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503824200"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.06.005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.031"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00569-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3460659"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00147.2003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00319.2003"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)30046-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200420846"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19874"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214571"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200106143442403"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc5001.102111"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000903"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5469.1248"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405533200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003359900914"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1263"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.1076"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.21.e92"},{"key":"e_1_2_6_26_1","first-page":"90","volume-title":"Methods 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