{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:12:22Z","timestamp":1761988342744,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,12,9]],"date-time":"2010-12-09T00:00:00Z","timestamp":1291852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:p>\n                    Activation of the heterotrimeric G protein Gq causes cardiomyocyte hypertrophy\n                    <jats:italic>in vivo<\/jats:italic>\n                    and in cell models. Our previous studies have shown that responses to activated Gq in cardiomyocytes are mediated exclusively by phospholipase C\u03b21b (PLC\u03b21b), because only this PLC\u03b2 subtype localizes at the cardiac sarcolemma. In the current study, we investigated the proteins involved in targeting PLC\u03b2 1b to the sarcolemma in neonatal rat cardiomyocytes. PLC\u03b2 1b, but not PLC\u03b21a, coimmunoprecipitated with the high\u2010MW scaffolding protein SH3 and ankyrin repeat protein 3 (Shank3), as well as the known Shank3\u2010interacting protein \u03b1\u2010fodrin. The 32\u2010aa splice\u2010variant\u2010specific C\u2010terminal tail of PLC\u03b2 1b also associated with Shank3 and \u03b1\u2010fodrin, indicating that PLC\u03b2 1b binds\n                    <jats:italic>via<\/jats:italic>\n                    the C\u2010terminal sequence. Shank3 colocalized with PLC\u03b2 1b at the sarcolemma, and both proteins were enriched in the light membrane fractions. Knockdown of Shank3 using siRNA reduced PLC activation and downstream hypertrophic responses, demonstrating the importance of sarcolemmal localization for PLC signaling. These data indicate that PLC\u03b2 1b associates with a Shank3 complex at the cardiac sarcolemma\n                    <jats:italic>via<\/jats:italic>\n                    its splice\u2010variant\u2010specific C\u2010terminal tail. Sarcolemmmal localization is central to PLC activation and subsequent downstream signaling following Gq\u2010coupled receptor activation.\u2014Grubb, D. R., Iliades, P., Cooley, N., Yu, Y. L., Luo, J., Filtz, T. M., Woodcock, E. A. Phospholipase C\u03b21b associates with a Shank3 complex at the cardiac sarcolemma.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    25, 1040\u20131047 (2011).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-171470","type":"journal-article","created":{"date-parts":[[2010,12,9]],"date-time":"2010-12-09T23:46:49Z","timestamp":1291938409000},"page":"1040-1047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Phospholipase C\u03b2lb associates with a Shank3 complex at the cardiac sarcolemma"],"prefix":"10.1096","volume":"25","author":[{"given":"David R.","family":"Grubb","sequence":"first","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Iliades","sequence":"additional","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Cooley","sequence":"additional","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen Lin","family":"Yu","sequence":"additional","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieting","family":"Luo","sequence":"additional","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa M.","family":"Filtz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Oregon State University Corvallis Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Woodcock","sequence":"additional","affiliation":[{"name":"Baker International Diabetes Institute (IDI) Heart and Diabetes Institute Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,9]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_2_1","DOI":"10.1016\/S1050-1738(99)00004-3"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_1","DOI":"10.1126\/science.280.5363.574"},{"doi-asserted-by":"publisher","key":"e_1_2_6_4_1","DOI":"10.1096\/fj.09-133983"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_1","DOI":"10.1096\/fj.07-102558"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_1","DOI":"10.1016\/j.yjmcc.2009.08.020"},{"doi-asserted-by":"publisher","key":"e_1_2_6_7_1","DOI":"10.1111\/j.1399-3011.2005.00240.x"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_1","DOI":"10.1074\/jbc.M006635200"},{"doi-asserted-by":"publisher","key":"e_1_2_6_9_1","DOI":"10.1074\/jbc.M001410200"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_1","DOI":"10.1038\/sj.onc.1208593"},{"doi-asserted-by":"publisher","key":"e_1_2_6_11_1","DOI":"10.1074\/jbc.M410740200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"8240","DOI":"10.1016\/S0021-9258(17)37185-5","article-title":"Two forms of phospholipase C\u2010b 1 generated by alternative splicing","volume":"269","author":"Bahk Y. 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