{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T23:31:47Z","timestamp":1772148707350,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,11,23]],"date-time":"2010-11-23T00:00:00Z","timestamp":1290470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:p>\n                    5\u2010Lipoxygenase (5\u2010LO) is the essential enzyme for the biosynthesis of leukotrienes, important mediators of inflammation. This study investigated whether variants of 5\u2010LO exist in human leukocytes. 5\u2010LO mRNA isoforms that are consistent with alternative splicing were identified by RT\u2010PCR in a cell line or cell type\u2010specific pattern. All evaluated cells expressed mRNA containing all 14 exons of 5\u2010LO with the expected splicing sites. Individual isoforms that retained intron 10 (\u03b1\u201010), lacked exon 13 (\u0394\u201013), and lacked exons 10 and 13 (\u0394\u201010,13) or that lacked the first 96 base pairs of exon 10 (\u0394\u2010p10) were identified. Immunoreactive bands coeluting with the cloned \u03b1\u201010 and \u0394\u201013 isoforms were measured in primary neutrophils and in Raji cells. When expressed in HEK293 cells, alternative proteins were without catalytic activity. However, when coexpressed with the active full\u2010length 5\u2010LO, alternative isoforms significantly decreased the biosynthesis of 5\u2010LO products by up to 44%, as assessed by reverse\u2010phase HPLC analysis. Additionally, in stimulated neutrophils the full\u2010length active 5\u2010LO was detected by immunoblot in both nuclear and non\u2010nuclear compartments, while the \u0394\u201013 isoform was only detected in the nuclear fraction. These alternative 5\u2010LO isoforms may represent a new mechanism for the regulation of the 5\u2010LO pathway and lipid mediator biosynthesis.\u2014Boudreau, L. H., Bertin, J., Robichaud, P. P., Laflamme, M., Ouellette, R. J., Flamand, N., Surette, M. E. Novel 5\u2010lipoxygenase isoforms affect the biosynthesis of 5\u2010lipoxygenase products.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    25, 1097\u20131105 (2011).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-173856","type":"journal-article","created":{"date-parts":[[2010,11,23]],"date-time":"2010-11-23T22:59:17Z","timestamp":1290553157000},"page":"1097-1105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Novel 5\u2010lipoxygenase isoforms affect the biosynthesis of 5\u2010lipoxygenase products"],"prefix":"10.1096","volume":"25","author":[{"given":"Luc H.","family":"Boudreau","sequence":"first","affiliation":[{"name":"D\u00e9partement de Chimie et Biochimie Universit\u00e9 de Moncton Moncton New Brunswick Canada"},{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec Departement de Medecine et Facult\u00e9 de M\u00e9decine Universit\u00e9 Laval Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Bertin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie et Biochimie Universit\u00e9 de Moncton Moncton New Brunswick Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe P.","family":"Robichaud","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie et Biochimie Universit\u00e9 de Moncton Moncton New Brunswick Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Atlantic Cancer Research Institute Moncton New Brunswick Canada"},{"name":"Department of Fisheries and Oceans Canada Moncton New Brunswick Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney J.","family":"Ouellette","sequence":"additional","affiliation":[{"name":"Atlantic Cancer Research Institute Moncton New Brunswick Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Flamand","sequence":"additional","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec Departement de Medecine et Facult\u00e9 de M\u00e9decine Universit\u00e9 Laval Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc E.","family":"Surette","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Chimie et Biochimie Universit\u00e9 de Moncton Moncton New Brunswick Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,11,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199807163390302"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071371"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7228-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1161\/CIRCIMAGING.110.937169","article-title":"Treatment with 5\u2010lipoxygenase inhibitor VIA\u20102291 (Atreleuton) in patients with recent acute coronary syndrome","volume":"3","author":"Tardif J. C.","journal-title":"Circ. Cardiovasc Imaging"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1311"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa025079"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvp385"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.21.9852"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"7816","DOI":"10.1016\/S0021-9258(19)57008-9","article-title":"Transformation of arachidonic acid and homo\u2010gamma\u2010linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenases","volume":"251","author":"Borgeat P.","year":"1976","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.2353\/ajpath.2010.100322","article-title":"Novel lipid mediators and resolution mechanisms in acute inflammation: to resolve or not?","volume":"177","author":"Serhan C. N.","journal-title":"Am. J Pathol."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.37.21652"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7393"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.1935"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203053545417"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008203200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006136200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011205200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605150103"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb18012.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M506513200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2007.06.002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"25109","DOI":"10.1074\/jbc.R110.125880","article-title":"Location, location, location: compartmentalization of early events in leukotriene biosynthesis","volume":"13","author":"Newcomer M. E.","year":"2010","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2587"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.23.9073"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2005.11.008"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2006.04.005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.08.011"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2007.04.007"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107665200"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090100"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700033200"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.08.031"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00818.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1042\/bj3140733","article-title":"The human leukotriene A4 hydrolase gene is expressed in two alternatively spliced mRNA forms","volume":"314","author":"Jendraschak E.","year":"1996","journal-title":"Biochem. J."},{"key":"e_1_2_6_36_1","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g","volume":"97","author":"Boyum A.","year":"1968","journal-title":"Scand. J. Clin. Lab. Invest. Suppl."},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.62.2.250"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01000.x"},{"key":"e_1_2_6_39_1","first-page":"C2057\u2010C2064","article-title":"Activated lymphocytes increase expression of 5\u2010lipoxygenase and its activating protein in THP\u20101 cells","volume":"273","author":"Ring W. L.","year":"1997","journal-title":"Am. J. Physiol. Cell. Physiol."},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)87014-T"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)72018-4"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505991102"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805593200"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07859.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2776"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1645"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn073"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2008.01.010"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00894-002-0076-y"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.t01-1-00625.x"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060669"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1144346"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.10-173856","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.10-173856","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.10-173856","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:46:17Z","timestamp":1761669977000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.10-173856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,23]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1096\/fj.10-173856"],"URL":"https:\/\/doi.org\/10.1096\/fj.10-173856","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,23]]},"assertion":[{"value":"2010-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}