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To explore hTERT regulation\n                    <jats:italic>in vivo,we<\/jats:italic>\n                    engineered mice with a 160\u2010kb transgenic bacterial artificial chromosome (BAC) spanning the\n                    <jats:italic>hTERT<\/jats:italic>\n                    locus with a\n                    <jats:italic>ReniUa<\/jats:italic>\n                    luciferase (\n                    <jats:italic>Rluc<\/jats:italic>\n                    ) cassette downstream of its promoter. Analysis of multiple founder lines revealed that the\n                    <jats:italic>Rluc<\/jats:italic>\n                    expression profile from the transgenic hTERT reporter locus reproduced that of the native\n                    <jats:italic>hTERT<\/jats:italic>\n                    gene in all tissues and organs examined, demonstrating that genetic sequence determined the species\u2010specific developmental regulation of the\n                    <jats:italic>hTERT<\/jats:italic>\n                    gene and that mouse epigenetic and transcription machineries faithfully regulated hTERT transcription. Thus, these mice allowed detailed analyses of developmental hTERT regulation. Both the transgenic hTERT reporter and the endogenous\n                    <jats:italic>mTERT<\/jats:italic>\n                    locus were expressed in early embryonic stages, and their mRNA levels progressively decreased throughout embryonic and postnatal development. Whereas hTERT transcription was much lower than mTERT expression in most organs, it increased significantly during postnatal development of thymus, testis, and ovary. In testis, the\n                    <jats:italic>Rluc<\/jats:italic>\n                    mRNA was enriched in elongating spermatids of seminiferous tubules. Inaddition, the transcription of transgenic hTERT reporter, but surprisingly not the endogenous\n                    <jats:italic>mTERT<\/jats:italic>\n                    gene, was activated during Wnt1\u2010induced mammary tumorigenesis, allowing the monitoring of tumor development\n                    <jats:italic>via<\/jats:italic>\n                    noninvasive bioluminescent imaging. Collectively, our results demonstrate that the hTERT transgenic reporter system recapitulates the developmental regulation of the\n                    <jats:italic>hTERT<\/jats:italic>\n                    gene in a chromosomal position\u2010independent manner and serves as a legitimate model to explore telomerase regulation in the development of normal and neoplastic tissues\n                    <jats:italic>in vivo.\u2014<\/jats:italic>\n                    Jia, W., Wang, S., Horner, J. W., Wang, N., Wang, H., Gunther, E. J., DePinho, R. A., Zhu, J. A BAC transgenic reporter recapitulates\n                    <jats:italic>in vivo<\/jats:italic>\n                    regulation of human telomerase reverse transcriptase in development and tumorigenesis.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    25, 979\u2013989 (2011).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-173989","type":"journal-article","created":{"date-parts":[[2010,12,7]],"date-time":"2010-12-07T00:40:42Z","timestamp":1291682442000},"page":"979-989","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A BAC transgenic reporter recapitulates\n                    <i>in vivo<\/i>\n                    regulation of human telomerase reverse transcriptase in development and tumorigenesis"],"prefix":"10.1096","volume":"25","author":[{"given":"Wenwen","family":"Jia","sequence":"first","affiliation":[{"name":"Shaanxi Center for Stem Cell Engineering and Technology College of Veterinary Medicine Northwest Agriculture and Forestry (A&amp;F) University Shaanxi China"},{"name":"Department of Cellular and Molecular Physiology Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James W.","family":"Horner","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huayan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shaanxi Center for Stem Cell Engineering and Technology College of Veterinary Medicine Northwest Agriculture and Forestry (A&amp;F) University Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Gunther","sequence":"additional","affiliation":[{"name":"Jake Gittlen Cancer Research Foundation Pennsylvania State University College of Medicine Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald A.","family":"DePinho","sequence":"additional","affiliation":[{"name":"Belfer Institute for Applied Cancer Science Dana\u2010Farber Cancer Institute Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology Hershey Pennsylvania 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