{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T05:52:51Z","timestamp":1772689971556,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,8,12]],"date-time":"2011-08-12T00:00:00Z","timestamp":1313107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The cellular repertoire of importin (IMP) proteins that mediates nuclear import of transcription factors and chromatin remodeling agents is critical to processes such as differentiation and transformation. This study identifies for the first time independent roles for specific IMP\u03b1s in murine embryonic stem cells (mESCs), showing that mESC differentiation is accompanied by dynamic changes in the levels of transcripts encoding the\n                    <jats:italic>IMPs, IMP\u03b13, IMP\u03b14, IMP\u03b21<\/jats:italic>\n                    , and\n                    <jats:italic>IPO5.<\/jats:italic>\n                    Of these, only\n                    <jats:italic>IMP\u03b14<\/jats:italic>\n                    was maintained at higher levels in differentiating mESCs, correlating with the finding that IMP\u03b14 overexpression induced a significant decrease in Oct3\/4 protein levels compared to control transfections. In parallel, IMP\u03b14 protein showed a unique and striking shift in subcellular localization from the nucleus to the cytoplasm during differentiation, which is consistent with activation of a role in nuclear import of differentiation factors. Overexpression of a dominant\u2010negative IMP\u03b12 isoform, when assessed against adjacent untransfected or IMP\u03b12 transfected cells, led to both a significant reduction in endogenous Oct3\/4 protein levels and inhibition of Oct3\/4 nuclear localization, suggesting that IMP\u03b12\u2010mediated delivery of Oct3\/4 to the nucleus contributes directly to maintenance of mESC pluripotency. These findings implicate IMP\u03b12 and IMP\u03b14 in specific but distinct roles in the fate choice between pluripotency and commitment to differentiation.\u2014Young, J. C., Major, A. T., Miyamoto, Y., Loveland, K. L., Jans, D. A. Distinct effects of importin \u03b12 and \u03b14 on Oct3\/4 localization and expression in mouse embryonic stem cells.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    25, 3958\u20133965 (2011).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.10-176941","type":"journal-article","created":{"date-parts":[[2011,8,12]],"date-time":"2011-08-12T21:12:07Z","timestamp":1313183527000},"page":"3958-3965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Distinct effects of importin \u03b12 and \u03b14 on Oct3\/4 localization and expression in mouse embryonic stem cells"],"prefix":"10.1096","volume":"25","author":[{"given":"Julia C.","family":"Young","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Biotechnology and Development Monash University  Clayton Victoria Australia"},{"name":"Department of Biochemistry and Molecular Biology Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew T.","family":"Major","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Biotechnology and Development Monash University  Clayton Victoria Australia"},{"name":"Department of Anatomy and Developmental Biology Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Biotechnology and Development Monash University  Clayton Victoria Australia"},{"name":"Department of Biochemistry and Molecular Biology Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate L.","family":"Loveland","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Biotechnology and Development Monash University  Clayton Victoria Australia"},{"name":"Department of Biochemistry and Molecular Biology Monash University  Clayton Victoria Australia"},{"name":"Department of Anatomy and Developmental Biology Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Jans","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Biotechnology and Development Monash University  Clayton Victoria Australia"},{"name":"Department of Biochemistry and Molecular Biology Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2165"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-05-0275"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-02-0069"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(04)00647-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.07.016"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2009.05.003"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00079-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/377246a0"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(200006)22:6<532::AID-BIES6>3.0.CO;2-O"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1142204"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00437.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20289"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20569"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1336.018"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","unstructured":"Loveland K. 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