{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T02:30:57Z","timestamp":1794537057279,"version":"4.9.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T00:00:00Z","timestamp":1317772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We tested the principle of treating malignant ovarian tumors by vaccination against their ectopically expressed protein, zona pellucida glycoprotein (ZP) 3, using as the experimental model the granulosa cell tumors that develop in transgenic mice expressing the simian virus 40 T\u2010antigen under the inhibin\u2010\u03b1 promoter (inh\u03b1\/Tag). We found high ZP3 expression in granulosa cell tumors of the transgenic mice, in human surface ovarian cancer and granulosa cell lines, and in human granulosa cell tumors and their metastases. Early preventive immunization (between 2 and 5.5 mo of age) of transgenic mice with recombinant human (rh) ZP3 prevented ovarian tumorigenesis, and delayed therapeutic immunization (between 4.5 and 7 mo) reduced weights of existing tumors by 86 and 75%, respectively (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001), compared to vehicle\u2010treated control mice. No objective side effects of the immunizations were observed. Liver metastases were found in nontreated\/vehicle\u2010treated controls (\n                    <jats:italic>n<\/jats:italic>\n                    =7\/39), but none following active rhZP3 immunizations (\n                    <jats:italic>n<\/jats:italic>\n                    =0\/36;\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05). Immunization with rhZP3 was highly effective, as demonstrated by the induction of anti\u2010ZP3 antibodies, as well as proliferative responses to the ZP3 antigen. These results signal rhZP3 immunization as a novel strategy to be developed for the immunotherapy of ovarian granulosa cell tumors, as well as for that of other malignancies that may express ZP3.Rahman, N. A., Coelingh Bennink, H.J. T., Chrusciel, M., Sharp, V., Zimmerman, Y., Dina, R., Li, X., Ellonen, A., Rivero\u2010M\u00fcller, A., Dilworth, S., Ghaem\u2010Maghami, S., Vainio, O., Huhtaniemi, I. A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 324\u2013333 (2012).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.11-192468","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T01:44:27Z","timestamp":1317865467000},"page":"324-333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3"],"prefix":"10.1096","volume":"26","author":[{"given":"Nafis A.","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Physiology University of Turku  Turku Finland"},{"name":"Department of Cell Biology Florida International University College of Medicine  Miami Florida USA"},{"name":"Department of Human Molecular Genetics Florida International University College of Medicine  Miami Florida USA"},{"name":"Department of Obstetrics and Gynecology Florida International University College of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herjan J. T. Coelingh","family":"Bennink","sequence":"additional","affiliation":[{"name":"Pantarhei Bioscience B.V.  Zeist The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Chrusciel","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Sharp","sequence":"additional","affiliation":[{"name":"Department of Surgery and Cancer Institute of Reproductive and Developmental Biology, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Pantarhei Bioscience B.V.  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