{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:42:09Z","timestamp":1793691729963,"version":"4.5.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,10,31]],"date-time":"2011-10-31T00:00:00Z","timestamp":1320019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003921","name":"Department of Health, Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012618","name":"National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012618","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Toll\u2010like receptors (TLRs) are important molecules involved in the activation of innate and subsequent development of adaptive immunity. TLRs are ligated by exogenous ligands from pathogens and by endogenous ligands released in inflammatory diseases. Activation of TLR leads to activation of NF\u2010\u03baB and release of proinflammatory cytokines, such as IL\u20106 and TNF\u2010\u03b1. TLRs play an important role in the pathogenesis of renal diseases. Increased expression of TLRs have been associated with ischemic kidney damage, acute kidney injury, end\u2010stage renal failure, acute renal transplant rejection, and delayed allograft function. OPN301 is a mouse anti\u2010human TLR2 antibody that cross\u2010reacts with mouse TLR2. We show that inhibition of TLR2 promotes graft function in an isograft model of renal transplantation. Recipient mice were treated intravenously with OPN301 before reperfusion of the transplanted kidney that had been subjected to 30 min of cold ischemia. After 5 d, the residual native kidney was removed, and renal transplant function was assessed 24 h later by measurement of blood urea nitrogen. Renal function in both saline\u2010 and isotype\u2010treated mice was similar, with significant improvement in OPN301\u2010treated mice (isotypetreated\n                    <jats:italic>vs.<\/jats:italic>\n                    OPN301\u2010treated: 33.9\u00b13.2\n                    <jats:italic>vs.<\/jats:italic>\n                    19.8\u00b11.9 \u03bcM;\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.01). The histopathological appearance corresponded with renal functional results. In OPN301\u2010treated recipients, renal structure was well preserved, whereas in the saline\u2010treated group, tubular injury was severe, with marked tubular thinning, epithelial shedding, cast formation and necrosis. Inhibition of TLR2 also leads to a decrease in C3d deposition, although it is unclear whether this is due directly to TLR2 inhibition or a decrease in renal inflammation. This study shows that inhibition of TLR2 with a therapeutic agent (OPN301) provides significant protection from ischemia\/reperfusion injury in a model of kidney transplantation.\u2014Farrar, C. A., Keogh, B., McCormack, W., O'Shaughnessy, A., Parker, A., Reilly, M., Sacks, S. H. Inhibition of TLR2 promotes graft function in a murine model of renal transplant ischemia\u2010reperfusion injury.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 799\u2013807 (2012). www.fasebj.org\n                  <\/jats:p>","DOI":"10.1096\/fj.11-195396","type":"journal-article","created":{"date-parts":[[2011,10,31]],"date-time":"2011-10-31T23:25:22Z","timestamp":1320103522000},"page":"799-807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Inhibition of TLR2 promotes graft function in a murine model of renal transplant ischemia\u2010reperfusion injury"],"prefix":"10.1096","volume":"26","author":[{"given":"Conrad A.","family":"Farrar","sequence":"first","affiliation":[{"name":"Medical Research Council Centre for Transplantation, Division of Transplantation Immunology and Mucosal Biology King's College London School of Medicine at Guy's, King's College, and St. Thomas' Hospitals, Guy's Hospital  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Keogh","sequence":"additional","affiliation":[{"name":"Opsona Therapeutics, Institute of Molecular Medicine Trinity Centre for Health Sciences, Trinity College Dublin, St. James' Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"McCormack","sequence":"additional","affiliation":[{"name":"Opsona Therapeutics, Institute of Molecular Medicine Trinity Centre for Health Sciences, Trinity College Dublin, St. James' Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisling","family":"O'Shaughnessy","sequence":"additional","affiliation":[{"name":"Opsona Therapeutics, Institute of Molecular Medicine Trinity Centre for Health Sciences, Trinity College Dublin, St. James' Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Parker","sequence":"additional","affiliation":[{"name":"Opsona Therapeutics, Institute of Molecular Medicine Trinity Centre for Health Sciences, Trinity College Dublin, St. James' Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Reilly","sequence":"additional","affiliation":[{"name":"Opsona Therapeutics, Institute of Molecular Medicine Trinity Centre for Health Sciences, Trinity College Dublin, St. James' Hospital  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven H.","family":"Sacks","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Transplantation, Division of Transplantation Immunology and Mucosal Biology King's College London School of Medicine at Guy's, King's College, and St. Thomas' Hospitals, Guy's Hospital  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,31]]},"reference":[{"key":"e_1_2_25_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e32803fb767"},{"key":"e_1_2_25_3_1","first-page":"406","article-title":"Immunosuppressive strategies in organ transplantation in the light of innate immunity","volume":"4","author":"Land W. 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