{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:09:44Z","timestamp":1773281384712,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,11,30]],"date-time":"2011-11-30T00:00:00Z","timestamp":1322611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP\u201068930"],"award-info":[{"award-number":["MOP\u201068930"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    Leukotrienes have been implicated in the pathogenesis of degenerative diabetic retinopathy, with research focusing primarily on leukotriene B\n                    <jats:sub>4<\/jats:sub>\n                    , with little attention devoted to the cysteinyl leukotrienes (cysLTs), which act through cysLT receptors (CysLT1R and CysLT\n                    <jats:sub>2<\/jats:sub>\n                    R). We demonstrate here the presence of CysLT\n                    <jats:sub>2<\/jats:sub>\n                    R in pericytes and endothelial cells of superficial retinal vasculature using an indirect assay by assessment of \u03b2\u2010galactosidase expression in CysLT\n                    <jats:sub>2<\/jats:sub>\n                    R\u2010knockout (KO) mice. Retinal damage was induced in KO and wild\u2010type (WT) mice using an established oxygen\u2010induced retinopathy (OIR) model. CysLT\n                    <jats:sub>2<\/jats:sub>\n                    R expression following OIR was intensely up\u2010regulated compared to sham\u2010treated controls. Staining with\n                    <jats:italic>Griffonia simplicifolia<\/jats:italic>\n                    lectin revealed enhanced tissue damage (as assessed by vasoobliteration\/vasoproliferation) in KO mice compared to WT controls, yet the opposite was true with respect to retinal edema. However, vascular endothelial growth factor receptor 1 (VEGFR1) transcripts were increased by OIR similarly with respect to genotype. Intravitreal application of exogenous cysLTs elicited greater vasculature leakage (assessed\n                    <jats:italic>ex vivo<\/jats:italic>\n                    ) in eyes from WT mice compared to KO mice. While mRNA encoding enzymes for various components of the leukotriene cascade were detected in sham\u2010 and OIR\u2010treated retinas, only prostaglandins and hydroxyeicosatetraenoic acids, but not leukotrienes, were detected in A23187\u2010treated retina preparations. Together, these results implicate the CysLT\n                    <jats:sub>2<\/jats:sub>\n                    R in the progression of ischemic retinopathy.\u2014Barajas\u2010Espinosa, A., Ni, N. C., Yan, D., Zarini, S., Murphy, R. C., Funk, C. D. The cysteinyl leukotriene 2 receptor mediates retinal edema and pathological neovascularization in a murine model of oxygen\u2010induced retinopathy.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 1100\u20101109 (2012).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.11-195792","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T05:23:36Z","timestamp":1322803416000},"page":"1100-1109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The cysteinyl leukotriene 2 receptor mediates retinal edema and pathological neovascularization in a murine model of oxygen\u2010induced retinopathy"],"prefix":"10.1096","volume":"26","author":[{"given":"Alma","family":"Barajas\u2010Espinosa","sequence":"first","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University Kingston Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan C.","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University Kingston Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University Kingston Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Zarini","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Colorado\u2013Denver Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Colorado\u2013Denver Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin D.","family":"Funk","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University Kingston Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02374-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6301011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/21658"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003490200"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601341"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808993105"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3887"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-113274"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.02.041"},{"key":"e_1_2_7_11_1","first-page":"1471","article-title":"Ischemia and reperfusion\u2010induced histologic changes in the rat retina. 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