{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T23:18:12Z","timestamp":1793920692448,"version":"4.8.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T00:00:00Z","timestamp":1327449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["107744"],"award-info":[{"award-number":["107744"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>\n                    Most presently known breast cancer susceptibility genes have been linked to DSB repair. To identify novel markers that may serve as indicators for breast cancer risk, we performed DSB repair analyses using a case\u2010control design. Thus, we examined 35 women with defined familial history of breast and\/or ovarian cancer (first case group), 175 patients with breast cancer (second case group), and 245 healthy women without previous cancer or family history of breast cancer (control group). We analyzed DSB repair in peripheral blood lymphocytes (PBLs) by a GFP\u2010based test system using 3 pathway\u2010specific substrates. We found increases of microhomology\u2010mediated nonhomologous end joining (mmNHEJ) and nonconservative single\u2010strand annealing (SSA) in women with familial risk\n                    <jats:italic>vs.<\/jats:italic>\n                    controls (\n                    <jats:italic>P<\/jats:italic>\n                    =0.0001\u20100.0022) and patients with breast cancer\n                    <jats:italic>vs.<\/jats:italic>\n                    controls (\n                    <jats:italic>P<\/jats:italic>\n                    =0.0004\u20100.0042). Young age (&lt;50) at initial diagnosis of breast cancer, which could be indicative of genetic predisposition, was associated with elevated SSA using two different substrates, amounting to similar odds ratios (ORs=2.54\u20104.46,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0059\u20100.0095) as for familial risk (ORs=2.61\u20104.05,\n                    <jats:italic>P<\/jats:italic>\n                    =0.0007\u20100.0045). These findings and supporting validation data underscore the great potential of detecting distinct DSB repair activities in PBLs as method to estimate breast cancer susceptibility beyond limitations of genotyping and to predict responsiveness to therapeutics targeting DSB repair\u2010dysfunctional tumors.\u2014Keimling, M., Deniz, M., Varga, D., Stahl, A., Schrezenmeier, H., Kreienberg, R., Hoffmann, I., K\u00f6nig, J., Wiesm\u00fcller, L. The power of DNA double\u2010strand break (DSB) repair testing to predict breast cancer susceptibility.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 2094\u20102104 (2012).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.11-200790","type":"journal-article","created":{"date-parts":[[2012,1,26]],"date-time":"2012-01-26T00:07:45Z","timestamp":1327536465000},"page":"2094-2104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["The power of DNA double\u2010strand break (DSB) repair testing to predict breast cancer susceptibility"],"prefix":"10.1096","volume":"26","author":[{"given":"Marlen","family":"Keimling","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Ulm University 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