{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:40:12Z","timestamp":1790052012626,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,7,5]],"date-time":"2012-07-05T00:00:00Z","timestamp":1341446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30 CA082709"],"award-info":[{"award-number":["P30 CA082709"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA95042"],"award-info":[{"award-number":["R01CA95042"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ascites in epithelial ovarian cancer (EOC) promotes tumor development by mechanisms that are incompletely understood. Lysophosphatidic acid (LPA), a major tumor\u2010promoting factor in EOC ascites, is an enzymatic product of autotaxin (ATX) and phospholipase A\n                    <jats:sub>2<\/jats:sub>\n                    (PLA\n                    <jats:sub>2<\/jats:sub>\n                    )enzymes. The contribution of PLA\n                    <jats:sub>2<\/jats:sub>\n                    activities to ovarian tumorigenesis was investigated. The quantitative measurement of PLA\n                    <jats:sub>2<\/jats:sub>\n                    activities in ascites and tissues, as well as assay conditions selective for PLA\n                    <jats:sub>2<\/jats:sub>\n                    subtypes, were optimized and validated. PLA\n                    <jats:sub>2<\/jats:sub>\n                    activities correlated with tumor\u2010promoting activates in cell\u2010based and\n                    <jats:italic>in vivo<\/jats:italic>\n                    assays. High activities consistent with both cytosolic and calcium\u2010independent PLA\n                    <jats:sub>2<\/jats:sub>\n                    were found in human EOC ascites for the first time. Elevated PLA\n                    <jats:sub>2<\/jats:sub>\n                    and ATX activities were also observed in EOC compared to benign tumors and normal tissues. Cell\u2010free and vesicle\u2010free (S4) human EOC ascites potently promoted proliferation, migration, and invasion of human EOC cells in a PLA\n                    <jats:sub>2<\/jats:sub>\n                    \u2010dependent manner. LPA mediated a significant part of the cell\u2010stimulating effects of ascites. S4 ascites stimulated tumorigenesis\/metastasis\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and methyl arachidonyl fluorophosphonate was highly effective in inhibiting EOC metastasis in mouse xenograft models. PLA\n                    <jats:sub>2<\/jats:sub>\n                    activity was found in conditioned media from both EOC cells and macrophages. Collectively, our work implies that PLA\n                    <jats:sub>2<\/jats:sub>\n                    activity is a potential marker and therapeutic target in EOC.\u2014Cai, Q., Zhao, Z., Antalis, C., Yan, L., Del Priore, G., Hamed, A. H., Stehman, F. B., Schilder, J. M., Xu, Y. Elevated and secreted phospholipase A\n                    <jats:sub>2<\/jats:sub>\n                    activities as new potential therapeutic targets in human epithelial ovarian cancer.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 3306\u20133320 (2012).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.12-207597","type":"journal-article","created":{"date-parts":[[2012,7,6]],"date-time":"2012-07-06T02:34:02Z","timestamp":1341542042000},"page":"3306-3320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Elevated and secreted phospholipase A\n                    <sub>2<\/sub>\n                    activities as new potential therapeutic targets in human epithelial ovarian cancer"],"prefix":"10.1096","volume":"26","author":[{"given":"Qingchun","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenwen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caryl","family":"Antalis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libo","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Del Priore","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Hassan","family":"Hamed","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick B.","family":"Stehman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne M.","family":"Schilder","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568005310303010023"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210093"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0005583","article-title":"Lysophosphatidic acid\u2010induced transcriptional profile represents serous epithelial ovarian carcinoma and worsened prognosis","volume":"4","author":"Murph M. 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