{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:11:42Z","timestamp":1791295902710,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T00:00:00Z","timestamp":1357257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>\n                    Growth hormone (GH) is a major anabolic hormone and the primary regulator of organism growth. Its transcription is triggered by GH\u2010releasing hormone (GHRH) through the transcription factor cAMP response element\u2010binding protein (CREB) and by caloric intake. In contrast, the deacetylase Sirt1 is activated by caloric restriction. Therefore, the present study investigates how Sirt1 affects CREB function and GH synthesis. Sirt1 pharmacological activation with resveratrol (IC\n                    <jats:sub>50<\/jats:sub>\n                    =87 \u03bcM) suppressed GHRH\u2010induced GH secretion from rat anterior pituitary cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    , while vehicle controls showed no effect. Resveratrol's effects were abolished after knocking down Sirt1 with RNA interference, but not in control scrambled siRNA\u2010transfected rat somatotrophs, confirming the Sirt1 specificity. Sirt1 activation and overexpression suppressed forskolin\u2010induced CREB\u2010Ser\n                    <jats:sup>133<\/jats:sup>\n                    phosphorylation, but no effect was seen with vehicle and empty plasmid controls. The deacetylase\u2010dead mutant Sirt1 retained CREB\u2010Ser\n                    <jats:sup>133<\/jats:sup>\n                    phosphorylation by keeping protein phosphatase protein phosphatase 1 activity low. Sirt1 activation suppressed glycogen synthase kinase 3 \u03b2 acetylation, and a mutation on the GSK3\u03b2\u2010Lys\n                    <jats:sup>205<\/jats:sup>\n                    residue mimicking a hypoacetylated form revealed increased activity. In summary, this is a novel mechanism through which Sirt1 intercepts the cAMP pathway by suppressing CREB transcriptional activation, resulting in decreased GH synthesis.\u2014Monteserin\u2010Garcia, J., Al\u2010Massadi, O., Seoane, L. M., Alvarez, C. V., Shan, B., Stalla, J., Paez\u2010Pereda, M., Casanueva, F. F., Stalla, G. K., Theodoropoulou, M. Sirt1 inhibits the transcription factor CREB to regulate pituitary growth hormone synthesis.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    27, 1561\u20131571 (2013).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.12-220129","type":"journal-article","created":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T23:07:26Z","timestamp":1357340846000},"page":"1561-1571","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Sirt1 inhibits the transcription factor CREB to regulate pituitary growth hormone synthesis"],"prefix":"10.1096","volume":"27","author":[{"given":"Jose","family":"Monteserin\u2010Garcia","sequence":"first","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Al\u2010Massadi","sequence":"additional","affiliation":[{"name":"Endocrine Physiopathology Group University Hospital Complex of Santiago de Compostela\/Servizo Galego de Sa\u00fade (SERGAS) Santiago de Compostela Spain"},{"name":"Department of Medicine University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa M.","family":"Seoane","sequence":"additional","affiliation":[{"name":"Endocrine Physiopathology Group University Hospital Complex of Santiago de Compostela\/Servizo Galego de Sa\u00fade (SERGAS) Santiago de Compostela Spain"},{"name":"Department of Medicine University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara V.","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Medicas e Instituto de Investigaciones Sanitarias (CIMUS\u2010IDIS) Department of Physiology School of Medicine University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Stalla","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Paez\u2010Pereda","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe F.","family":"Casanueva","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Santiago de Compostela Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter K.","family":"Stalla","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marily","family":"Theodoropoulou","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Max Planck Institute of Psychiatry Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-8-2-115"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2008-0027"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1191"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39375"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(04)63006-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.151S023"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001845"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/345829a0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2831625"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90037-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90038-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/S0021-9258(17)42098-9","article-title":"Two CGTCA motifs and a GHF1\/Pit1 binding site mediate cAMP\u2010dependent protein kinase A regulation of human growth hormone gene expression in rat anterior pituitary GC cells","volume":"269","author":"Shepard A. 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