{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T08:37:07Z","timestamp":1776760627510,"version":"3.51.2"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    Imprinted genes are monoallelically expressed according to the parent of origin and are critical for proper placental and embryonic development. Disruption of methylation patterns at imprinted loci resulting in loss of imprinting (LOI) may lead to serious imprinting disorders (\n                    <jats:italic>e.g.<\/jats:italic>\n                    , Beckwith\u2010Wiedemann syndrome) and is described in some cancers (\n                    <jats:italic>e.g.<\/jats:italic>\n                    , Wilms' tumor). As most research has focused on children with cancer or other abnormal phenotypes, the imprinting status in healthy infants at birth has not been characterized. We examined the prevalence of\n                    <jats:italic>H19<\/jats:italic>\n                    and\n                    <jats:italic>IGF2<\/jats:italic>\n                    LOI at birth by allele\u2010specific expression assays analysis on 114 human individuals. Overall expression and methylation analyses were performed on a subset of samples. We found that LOI of\n                    <jats:italic>H19<\/jats:italic>\n                    was observed for 4% of individuals in cord blood and 3.3% in placenta, and for\n                    <jats:italic>IGF2<\/jats:italic>\n                    of 22% of individuals in the cord blood and 0% in placenta. Interestingly, LOI status did not correspond to aberrant methylation levels of the imprinted DMRs or with changes in overall gene expression for the majority of individuals. Our observations suggest that LOI is present in phenotypically healthy infants. Determining a \u201cnormal\u201d human epigenotype range is important for discovering factors required to maintain a healthy pregnancy and embryonic development.\u2014Rancourt, R. C., Harris, H. R., Barault, L., Michels, K. B., The prevalence of loss of imprinting of\n                    <jats:italic>H19<\/jats:italic>\n                    and\n                    <jats:italic>IGF2<\/jats:italic>\n                    at birth. FASEB J. 27, 3335\u20133343 (2013).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.12-225284","type":"journal-article","created":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T23:51:01Z","timestamp":1366933861000},"page":"3335-3343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The prevalence of loss of imprinting of\n                    <i>H19<\/i>\n                    and\n                    <i>IGF2<\/i>\n                    at birth"],"prefix":"10.1096","volume":"27","author":[{"given":"Rebecca C.","family":"Rancourt","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Epidemiology Center Brigham and Women's Hospital Harvard Medical School Boston Massachusetts USA"},{"name":"Department of Epidemiology Harvard School of Public Health Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holly R.","family":"Harris","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Epidemiology Center Brigham and Women's Hospital Harvard Medical School Boston Massachusetts USA"},{"name":"Division of Nutritional Epidemiology National Institute for Environmental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludovic","family":"Barault","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Epidemiology Center Brigham and Women's Hospital Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin B.","family":"Michels","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Epidemiology Center Brigham and Women's Hospital Harvard Medical School Boston Massachusetts USA"},{"name":"Department of Epidemiology Harvard School of Public Health Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,25]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"R47","DOI":"10.1093\/hmg\/ddi114","article-title":"Epigenetic reprogramming in mammals","volume":"14","author":"Morgan H. 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