{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:00:24Z","timestamp":1789822824760,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T00:00:00Z","timestamp":1365465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013888","name":"Heart and Stroke Foundation of British Columbia and Yukon","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>\n                    Coxsackievirus B3 (CVB3) is a causative agent of viral myocarditis, hepatitis, pancreatitis, and meningitis in humans. The adenosine\u2010uridine (AU)\u2010rich element RNA binding factor 1 (AUF1) is an integral component in the regulation of gene expression. AUF1 destabilizes mRNAs and targets them for degradation by binding to AU\u2010rich elements in the 3\u2032 untranslated region (UTR) of mRNAs. The 3\u2032\u2010UTR of the CVB3 genome contains canonical AU\u2010rich sequences, raising the possibility that CVB3 RNA may also be subjected to AUF1\u2010mediated degradation. Here, we reported that CVB3 infection led to cytoplasmic redistribution and cleavage of AUF1. These events are independent of CVB3\u2010induced caspase activation but require viral protein production. Overexpression of viral protease 2A reproduced CVB3\u2010induced cytoplasmic redistribution of AUF1, while\n                    <jats:italic>in vitro<\/jats:italic>\n                    cleavage assay revealed that viral protease 3C contributed to AUF1 cleavage. Furthermore, we showed that knockdown of AUF1 facilitated viral RNA, protein, and progeny production, suggesting an antiviral property for AUF1 against CVB3 infection. Finally, an immunoprecipitation study demonstrated the physical interaction between AUF1 and the 3\u2032\u2010UTR of CVB3, potentially targeting CVB3 genome toward degradation. Together, our results suggest that cleavage of AUF1 may be a strategy employed by CVB3 to enhance the stability of its viral genome.\u2014Wong, J., Si, X., Angeles, A., Zhang, J., Shi, J., Fung, G., Jagdeo, J., Wang, T., Zhong, Z., Jan, E., Luo, H. Cytoplasmic redistribution and cleavage of AUF1 during coxsackievirus infection enhance the stability of its viral genome.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    27, 2777\u20102787 (2013).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.12-226498","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T00:44:38Z","timestamp":1365554678000},"page":"2777-2787","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Cytoplasmic redistribution and cleavage of AUF1 during coxsackievirus infection enhance the stability of its viral genome"],"prefix":"10.1096","volume":"27","author":[{"given":"Jerry","family":"Wong","sequence":"first","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoning","family":"Si","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arkhjamil","family":"Angeles","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyan","family":"Shi","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Fung","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julienne","family":"Jagdeo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohua","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Microbiology Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Jan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Luo","sequence":"additional","affiliation":[{"name":"James Hogg Research Center Providence Heart and Lung Institute St. Paul's Hospital Vancouver British Columbia Canada"},{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia 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