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The effect of local depot C6\u2032 or C6 was evaluated in mice with a priapic phenotype due to double neuronal and endothelial NO synthase deletion\n                    <jats:italic>\n                      (dNOS\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                      )\n                    <\/jats:italic>\n                    or human sickle hemoglobin transgenic expression (Sickle). Changes in NO signaling molecules and reactive oxygen species (ROS) surrogates were assessed by Western blot. The physiological response after C6\u2032 treatment was assessed using an established model of electrically stimulated penile erection. C6\u2032 generated NO, increased cGMP, and dose dependently increased NO metabolites. C6\u2032 treatment reversed abnormalities in key penile erection signaling molecules, including phosphodiesterase type 5, phosphorylated endothelial nitric oxide synthase, and phosphorylated vasodilator\u2010stimulated phosphoprotein. In Sickle mice, C6\u2032 also attenuated the increased ROS markers gp91\n                    <jats:sup>phox<\/jats:sup>\n                    , 4\u2010hydroxynonenal, and 3\u2010nitrotyrosine. Finally, C6\u2032 corrected the excessive priapic erection response of\n                    <jats:italic>\n                      dNOS\n                      <jats:sup>\u2010\/\u2010<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Exogenous sustained NO release from C6\u2032 corrects pathological erectile signaling in mouse models of priapism and suggests novel approaches to human therapy.\u2014Lagoda, G., Sezen, S. F., Hurt, K. J., Cabrini, M. R., Mohanty, D. K., Burnett, A. L. Sustained nitric oxide (NO)\u2010releasing compound reverses dysregulated NO signal transduction in priapism.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    28, 76\u201384 (2014).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-228817","type":"journal-article","created":{"date-parts":[[2013,9,28]],"date-time":"2013-09-28T01:13:59Z","timestamp":1380330839000},"page":"76-84","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Sustained nitric oxide (NO)\u2010releasing compound reverses dysregulated NO signal transduction in priapism"],"prefix":"10.1096","volume":"28","author":[{"given":"Gwen","family":"Lagoda","sequence":"first","affiliation":[{"name":"The James Buchanan Brady Urological Institute Baltimore Maryland USA"},{"name":"Department of Urology The Johns Hopkins School of Medicine Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sena F.","family":"Sezen","sequence":"additional","affiliation":[{"name":"The James Buchanan Brady Urological Institute Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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