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To investigate this, we inactivated the\n                    <jats:italic>Wnt4<\/jats:italic>\n                    gene by crossing\n                    <jats:italic>Amhr2Cre<\/jats:italic>\n                    and doxycycline\u2010inducible Rosa\n                    <jats:sup>rtTA<\/jats:sup>\n                    \u2010knock\u2010in\n                    <jats:italic>Cre<\/jats:italic>\n                    mice with mice carrying a floxed\n                    <jats:italic>Wnt4<\/jats:italic>\n                    allele and used a novel\n                    <jats:italic>\n                      Wnt4\n                      <jats:sup>mCherry<\/jats:sup>\n                    <\/jats:italic>\n                    \u2010knock\u2010in mouse. In these models, ovarian folliculogenesis was compromised, and female fertility was severely reduced, and\n                    <jats:italic>Wnt4<\/jats:italic>\n                    deficiency eventually led to premature ovarian failure. These anomalies were associated with cell polarity defects in the follicle. Within the follicle, laminin and type IV collagen assembled ectopic basement membrane\u2013like structures, the cell adherens junction components N\u2010cadherin and \u03b2\u2010catenin lost their polarized expression pattern, and expression of the gap junction protein connexin 43 was reduced by ~30% when compared with that of the controls. Besides these changes, expression of antimullerian hormone (\n                    <jats:italic>Amh<\/jats:italic>\n                    ) was inhibited in the absence of\n                    <jats:italic>Wnt4<\/jats:italic>\n                    signaling\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Consistent with this,\n                    <jats:italic>Wnt4<\/jats:italic>\n                    signaling up\u2010regulated\n                    <jats:italic>Amh<\/jats:italic>\n                    gene expression in KK1 cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Thus,\n                    <jats:italic>Wnt4<\/jats:italic>\n                    signaling is necessary during maturation of the ovarian follicles, where it coordinates expression of\n                    <jats:italic>Amh<\/jats:italic>\n                    , cell survival, and polarized organization of the follicular cells.\u2014Prunskaite\u2010Hyyrylainen, R., Shan, J., Railo, A., Heinonen, K. M., Miinalainen, I., Yan, W., Shen, B., Perreault, C., Vainio, S.J. Wnt4, a pleiotropic signal for controlling cell polarity, basement membrane integrity, and antimullerian hormone expression during oocyte maturation in the female follicle.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    28, 28\u20131568 (1581).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-233247","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T21:56:43Z","timestamp":1388095003000},"page":"1568-1581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Wnt4, a pleiotropic signal for controlling cell polarity, basement membrane integrity, and antimullerian hormone expression during oocyte maturation in the female follicle"],"prefix":"10.1096","volume":"28","author":[{"given":"Renata","family":"Prunskaite\u2010Hyyrylainen","sequence":"first","affiliation":[{"name":"Oulu Centre for Cell Matrix Research, Department of Medical Biochemistry and Molecular Biology Institute of Biomedicine and Biocenter Oulu Laboratory of Developmental Biology University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingdong","family":"Shan","sequence":"additional","affiliation":[{"name":"Oulu Centre for Cell Matrix Research, Department of Medical Biochemistry and Molecular Biology Institute of Biomedicine and Biocenter Oulu Laboratory of Developmental Biology University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"Railo","sequence":"additional","affiliation":[{"name":"Oulu Centre for Cell Matrix Research, Department of Medical Biochemistry and Molecular Biology Institute of Biomedicine and Biocenter Oulu Laboratory of 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