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The disordered N\u2010terminal parts are always present in FET fusion oncoproteins of sarcomas and leukemia. Mutations in FUS and TAF15 cause aggregation of FET proteins in neurological disorders. Here we used recombinant proteins in pulldown experiments and mass spectrometry to identify major interaction partners of the FET N\u2010terminal parts. We report that FUS, EWSR1, and TAF15 form homo\u2010 and heterocomplexes as major binding partners and identify an evolutionarily conserved N\u2010terminal motif (FETBM1) that is required for this interaction. The binding is RNA and DNA independent and robust up to 1 M of NaCl. The localization of FETBM1 and its target sequences supports a simple model for FET protein aggregation as reported in neurological disorders such as amyotrophic lateral sclerosis, frontotemporal dementia, and essential tremor. The FETBM1 localization also explains the binding of normal full\u2010length FET proteins to their oncogenic fusion proteins.\u2014Thomsen, C., Grundevik, P., Elias, P., St\u00e5hlberg, A., \u00c5man, P., A conserved N\u2010terminal motif is required for complex formation between FUS, EWSR1, TAF15 and their oncogenic fusion proteins. FASEB J. 27, 4965\u20134974 (2013).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-234435","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T20:22:47Z","timestamp":1377289367000},"page":"4965-4974","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["A conserved N\u2010terminal motif is required for complex formation between FUS, EWSR1, TAF15 and their oncogenic fusion proteins"],"prefix":"10.1096","volume":"27","author":[{"given":"Christer","family":"Thomsen","sequence":"first","affiliation":[{"name":"Sahlgrenska Cancer Center Department of Pathology Institute of Biomedicine Sahlgrenska Academy University of Gothenburg Gothenburg 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