{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:58:26Z","timestamp":1792810706656,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T00:00:00Z","timestamp":1384992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01 HL114470"],"award-info":[{"award-number":["P01 HL114470"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>\n                    Pleural mesothelial cells (PMCs), which are derived from the mesoderm, exhibit an extraordinary capacity to undergo phenotypic changes during development and disease. PMC transformation and trafficking has a newly defined role in idiopathic pulmonary fibrosis (IPF); however, the contribution of Wilms' tumor 1 (Wt1)\u2010positive PMCs to the generation of pathognomonic myofibroblasts remains unclear. PMCs were obtained from IPF lung explants and healthy donor lungs that were not used for transplantation. Short hairpin Wt1\u2010knockdown PMCs (sh Wt1) were generated with Wt1 shRNA, and morphologic and functional assays were performed\n                    <jats:italic>in vitro.<\/jats:italic>\n                    Loss of Wt1 abrogated the PMC phenotype and showed evidence of mesothelial\u2010to\u2010mesenchymal transition (MMT), with a reduced expression of E\u2010cadherin and an increase in the profibrotic markers \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and fibronectin, along with increased migration and contractility, compared with that of the control. Migration of PMCs in response to active transforming growth factor (TGF)\u2010\u03b21 was assessed by live\u2010cell imaging with 2\u2010photon microscopy and 3D imaging, of Wt1\u2010EGFP transgenic mice. Lineage\u2010tracing experiments to map the fate of Wt1\n                    <jats:sup>+<\/jats:sup>\n                    PMCs in mouse lung in response to TGF\u2010\u03b21 were also performed by using a Cre\u2010loxP system. Our results, for the first time, demonstrate that Wt1 is necessary for the morphologic integrity of pleural membrane and that loss of Wt1 contributes to IPF\n                    <jats:italic>via<\/jats:italic>\n                    MMT of PMCs into a myofibroblast phenotype.\u2014Karki, S., Surolia, R., Hock, T. D., Guroji, P., Zolak, J. S., Duggal, R., Ye, T., Thannickal, V. J., Antony, V. B. Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    28, 1122\u20131131 (2014).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-236828","type":"journal-article","created":{"date-parts":[[2013,11,21]],"date-time":"2013-11-21T23:00:29Z","timestamp":1385074829000},"page":"1122-1131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis"],"prefix":"10.1096","volume":"28","author":[{"given":"Suman","family":"Karki","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranu","family":"Surolia","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas David","family":"Hock","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purusotham","family":"Guroji","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason S.","family":"Zolak","sequence":"additional","affiliation":[{"name":"Department of Neurobiology University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Duggal","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Neurobiology University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor J.","family":"Thannickal","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena B.","family":"Antony","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.1030055"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.157.1.9704130"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3410\/B2-78"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01212.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200602-018TK"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200602-205OC"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81270-2"},{"key":"e_1_2_8_9_1","first-page":"7","article-title":"Role for alpha3 integrin in EMT and pulmonary fibrosis","volume":"119","author":"Borok Z.","year":"2009","journal-title":"J. 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