{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T04:18:24Z","timestamp":1773548304307,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T00:00:00Z","timestamp":1377129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["909\/12"],"award-info":[{"award-number":["909\/12"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>\n                    In previous studies, the oligo\u2010acyl\u2010lysyl (OAK) C\n                    <jats:sub>12(\u03c97)<\/jats:sub>\n                    K\u2010\u03b2\n                    <jats:sub>12<\/jats:sub>\n                    added to cultures of gram\u2010positive bacteria exerted a bacteriostatic activity that was associated with membrane depolarization, even at high concentrations. Here, we report that multidrug\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    strains, unlike other gram\u2010positive species, have reverted to the sensitive phenotype when exposed to subminimal inhibitory concentrations (sub\u2010MICs) of the OAK, thereby increasing antibiotics potency by up to 3 orders of magnitude. Such chemosensitization was achieved using either cytoplasm or cell\u2010wall targeting antibiotics. Moreover, eventual emergence of resistance to antibiotics was significantly delayed. Using the mouse peritonitis\u2010sepsis model, we show that on single\u2010dose administration of oxacillin and OAK combinations, death induced by a lethal staphylococcal infection was prevented in a synergistic manner, thereby supporting the likelihood for synergism to persist under\n                    <jats:italic>in vivo<\/jats:italic>\n                    conditions. Toward illuminating the molecular basis for these observations, we present data arguing that sub\u2010MIC OAK interactions with the plasma membrane can inhibit proton\u2010dependent signal transduction responsible for expression and export of resistance factors, as demonstrated for \u03b2\u2010lactamase and PBP2a. Collectively, the data reveal a potentially useful approach for overcoming antibiotic resistance and for preventing resistance from emerging as readily as when bacteria are exposed to an antibiotic alone.\u2014Kaneti, G., Sarig, H., Marjieh, I., Fadia, Z., Mor, A., Simultaneous breakdown of multiple antibiotic resistance mechanisms in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . FASEB J. 27, 4834\u20104843 (2013).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-237610","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:10:19Z","timestamp":1377216619000},"page":"4834-4843","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Simultaneous breakdown of multiple antibiotic resistance mechanisms in\n                    <i>S. aureus<\/i>"],"prefix":"10.1096","volume":"27","author":[{"given":"Galoz","family":"Kaneti","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadar","family":"Sarig","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim","family":"Marjieh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaknoon","family":"Fadia","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amram","family":"Mor","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Engineering Technion\u2010Israel Institute of Technology Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1079\/9781845936570.0195","volume-title":"Antimicrobial Peptides: Discovery, Design and Novel Therapeutic Strategies","author":"Mayer M. 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