{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:54:58Z","timestamp":1791208498458,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T00:00:00Z","timestamp":1388016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>\n                    Factor H binding protein (fHbp) is one of the main antigens of the 4\u2010component meningococcus B (4CMenB) multicomponent vaccine against disease caused by serogroup B\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    (MenB). fHbp binds the complement down\u2010regulating protein human factor H (hfH), thus resulting in immune evasion. fHbp exists in 3 variant groups with limited cross\u2010protective responses. Previous studies have described the generation of monoclonal antibodies (mAbs) targeting variant\u2010specific regions of fHbp. Here we report for the first time the functional characterization of two mAbs that recognize a wide panel of fHbp variants and subvariants on the MenB surface and that are able to inhibit fHbp binding to hfH. The antigenic regions targeted by the two mAbs were accurately mapped by hydrogen\u2010deuterium exchange mass spectrometry (HDX\u2010MS), revealing partially overlapping epitopes on the N terminus of fHbp. Furthermore, while none of the mAbs had bactericidal activity on its own, a synergistic effect was observed for each of them when tested by the human complement serum bactericidal activity (hSBA) assay in combination with a second nonbactericidal mAb. The bases underlying fHbp variant cross\u2010reactivity, as well as inhibition of hfH binding and cooperativity effect observed for the two mAbs, are discussed in light of the mapped epitopes.\u2014Faleri, A, Santini, L., Brier, S., Pansegrau, W., Lo Surdo, P., Scarselli, M., Buricchi, F., Volpini, G., Genovese, A, van der Veen, S., Lea, S., Tang, C. M., Savino, S., Pizza, M., Finco, O., Norais, N., Masignani, V Two cross\u2010reactive monoclonal antibodies recognize overlapping epitopes on\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    factor H binding protein but have different functional properties.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    28, 28\u20131644 (1653).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-239012","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T21:56:43Z","timestamp":1388095003000},"page":"1644-1653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Two cross\u2010reactive monoclonal antibodies recognize overlapping epitopes on\n                    <i>Neisseria meningitidis<\/i>\n                    factor H binding protein but have different functional properties"],"prefix":"10.1096","volume":"28","author":[{"given":"Agnese","family":"Faleri","sequence":"first","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Santini","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Brier","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Pansegrau","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Lo","family":"Surdo","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Scarselli","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Buricchi","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Volpini","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Genovese","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stijn","family":"van der Veen","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Lea","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph M.","family":"Tang","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Savino","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oretta","family":"Finco","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Norais","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vega","family":"Masignani","sequence":"additional","affiliation":[{"name":"Research Center Novartis Vaccines and Diagnostics Srl Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/649209"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105033441807"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.5.4.855"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1586\/erv.10.116"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021911"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2088-2100.2004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1097\/INF.0b013e31826327e4","article-title":"Safety and immunogenicity of a meningococcal B bivalent rLP2086 vaccine in healthy toddlers aged 18\u201036 months: a phase 1 randomized\u2010controlled clinical trial","volume":"31","author":"Marshall H. 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