{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:59:30Z","timestamp":1791291570977,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:00:00Z","timestamp":1399507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP130561"],"award-info":[{"award-number":["MOP130561"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>\n                    Exosomes are extracellular vesicles that mediate intercellular communication and are involved in several biological processes. The objective of our study was to determine whether endogenous retrovirus group WE, member l (\n                    <jats:italic>ERVWE1<\/jats:italic>\n                    )\/syncytin\u20101 and endogenous retrovirus group FRD, member 1 (\n                    <jats:italic>ERVFRDE1<\/jats:italic>\n                    )\/ syncytin\u20102, encoded by human endogenous retrovirus (HERV) envelope (\n                    <jats:italic>env<\/jats:italic>\n                    ) genes, are present at the surface of exosomes produced by placenta\u2010derived villous cytotrophoblasts and whether they play a role in cellular uptake of exosomes. In addition, we sought to determine whether these proteins are present in various abundances in serum\u2010derived exosomes from normal pregnant women\n                    <jats:italic>vs.<\/jats:italic>\n                    women with preeclampsia (PE). Isolated exosomes were analyzed for their content by Western blot, a bead\u2010associated flow cytometry approach, and a syncytin\u20102 ELISA. Binding and uptake were tested through confocal and electron microscopy using the BeWo choriocarcinoma cell line. Quality control of exosome preparations consisted of detection of exosomal and nonexosomal markers. Exosome\u2013cell interactions were compared between cells incubated in the presence of control exosomes, syncytin\u20101 or syncytin\u20102\u2010deprived exosomes, or exosomes solely bearing the uncleaved forms of these HERV env proteins. From our data, we conclude that villous cytotrophoblast exosomes are positive for both env proteins and are rapidly taken up by BeWo cells in a syncytin\u20101\u2010 and syncytin\u20102\u2010dependent manner and that syncytin\u20102 is reduced in serum\u2010derived exosomes from women with PE when compared to exosomes from normal pregnant women.\u2014Vargas, A., Zhou, S., \u00c9thier\u2010Chiasson, M., Flipo, D., Lafond, J., Gilbert, C., Barbeau, B. Syncytin proteins incorporated in placenta exosomes are important for cell uptake and show variation in abundance in serum exosomes from patients with preeclampsia.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    28, 3703\u20133719 (2014).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.13-239053","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T23:57:26Z","timestamp":1399593446000},"page":"3703-3719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":191,"title":["Syncytin proteins incorporated in placenta exosomes are important for cell uptake and show variation in abundance in serum exosomes from patients with 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