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Common variants in\n                    <jats:italic>Hey2<\/jats:italic>\n                    are associated with Brugada syndrome. We hypothesized that absence of CHF1\/Hey2 would result in abnormal cellular electrical activity, altered cardiac conduction system (CCS) development, and increased arrhythmogenesis. We isolated neonatal CHF\/Hey2\u2010knockout (KO) cardiac myocytes and measured action potentials and ion channel subunit gene expression. We also crossed myocardial\u2010specific CHF1\/Hey2\u2010KO mice with cardiac conduction system LacZ reporter mice and stained for conduction system tissue. We also performed ambulatory ECG monitoring for arrhythmias and heart rate variability. Neonatal cardiomyocytes from CHF1\/Hey2\u2010KO mice demonstrate a 50% reduction in action potential\n                    <jats:italic>dV\/dT<\/jats:italic>\n                    , a 50\u201375% reduction in\n                    <jats:italic>SCN5A, KCNJ2<\/jats:italic>\n                    , and\n                    <jats:italic>CACNA1C<\/jats:italic>\n                    ion channel subunit gene expression, and an increase in delayed afterdepolarizations from 0\/min to 12\/min. CHF1\/Hey2 cKO CCS\u2010lacZ mice have a ~3\u2010fold increase in amount of CCS tissue. Ambulatory ECG monitoring showed no difference in cardiac conduction, arrhythmias, or heart rate variability. Wild\u2010type cells or animals were used in all experiments. CHF1\/Hey2 may contribute to Brugada syndrome by influencing the expression of\n                    <jats:italic>SCN5A<\/jats:italic>\n                    and formation of the cardiac conduction system, but its absence does not cause baseline conduction defects or arrhythmias in the adult mouse.\u2014Hartman, M. E., Liu, Y., Zhu, W.\u2010Z., Chien, W.\u2010M., Weldy, C. S., Fishman, G. I., Laflamme, M. A., Chin, M. T. Myocardial deletion of transcription factor CHF1\/Hey2 results in altered myocyte action potential and mild conduction system expansion but does not alter conduction system function or promote spontaneous arrhythmias.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 28, 3007\u20133015 (2014).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-251728","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T01:28:50Z","timestamp":1396315730000},"page":"3007-3015","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Myocardial deletion of transcription factor CHF1\/Hey2 results in altered myocyte action potential and mild conduction system expansion but does not alter conduction system function or promote spontaneous arrhythmias"],"prefix":"10.1096","volume":"28","author":[{"given":"Matthew E.","family":"Hartman","sequence":"first","affiliation":[{"name":"Division of Cardiology Department of Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggang","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Zhong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute for Stem Cells and Regenerative Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Ming","family":"Chien","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad S.","family":"Weldy","sequence":"additional","affiliation":[{"name":"Division of Cardiology Department of Medicine University of Washington Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn I.","family":"Fishman","sequence":"additional","affiliation":[{"name":"The Leon H. 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