{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T09:48:00Z","timestamp":1776505680709,"version":"3.51.2"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T00:00:00Z","timestamp":1423440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000850","name":"National Heart and Lung Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL080130"],"award-info":[{"award-number":["HL080130"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK063275"],"award-info":[{"award-number":["DK063275"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["J3398\u2010BW23"],"award-info":[{"award-number":["J3398\u2010BW23"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A20 protects against pathologic vascular remodeling by inhibiting the inflammatory transcription factor NF\u2010\u03baB. A20's function has been attributed to ubiquitin editing of receptor\u2010interacting protein 1 (RIP1) to influence activity\/stability. The validity of this mechanism was tested using a murine model of transplant vasculopathy and human cells. Mouse C57BL\/6 aortae transduced with adenoviruses containing A20 (or \u03b2\u2010galactosidase as a control) were allografted into major histocompatibility complex\u2010mismatched BALB\/c mice. Primary endothelial cells, smooth muscle cells, or transformed epithelial cells (all human) were transfected with wild\u2010type A20 or with catalytically inactive mutants as a control. NF\u2010\u03baB activity and intracellular localization of RIP1 was monitored by reporter gene assay, immunofluorescent staining, and Western blotting. Native and catalytically inactive versions of A20 had similar inhibitory effects on NF\u2010\u03baB activity (\u201070% vs. \u201076%;\n                    <jats:italic>P<\/jats:italic>\n                    &gt; 0.05). A20 promoted localization of RIP1 to insoluble aggresomes in murine vascular allografts and in human cells (53% vs.0%) without altering RIP1 expression, and this process was increased by the assembly of polyubiquitin chains (87% vs. 28%;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). A20 captures polyubiquitinated signaling intermediaries in insoluble aggresomes, thus reducing their bioavailability for downstream NF\u2010\u03baB signaling. This novel mechanism contributes to protection from vasculopathy in transplanted organs treated with exogenous A20.\u2014Enesa, K., Moll, H. P., Luong, L., Ferran, C., Evans, P. C. A20 suppresses vascular inflammation by recruiting proinflammatory signaling molecules to intracellular aggresomes.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 1869\u20101878 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-258533","type":"journal-article","created":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T23:30:40Z","timestamp":1423524640000},"page":"1869-1878","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A20 suppresses vascular inflammation by recruiting proinflammatory signaling molecules to intracellular aggresomes"],"prefix":"10.1096","volume":"29","author":[{"given":"Karine","family":"Enesa","sequence":"first","affiliation":[{"name":"British Heart Foundation Cardiovascular Sciences Unit Imperial College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herwig P.","family":"Moll","sequence":"additional","affiliation":[{"name":"Division of Vascular and Endovascular Surgery Center for Vascular Biology Research and the Transplant Institute Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le","family":"Luong","sequence":"additional","affiliation":[{"name":"British Heart Foundation Cardiovascular Sciences Unit Imperial College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Ferran","sequence":"additional","affiliation":[{"name":"Division of Vascular and Endovascular Surgery Center for Vascular Biology Research and the Transplant Institute Department of Surgery Beth Israel Deaconess Medical Center, Harvard Medical School Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul C.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Sciences University of Sheffield Sheffield United Kingdom"},{"name":"INSIGNEO Institute for In Silico Medicine, University of Sheffield Sheffield United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200301150-00002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3182419829"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4981com"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.04.029"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.8.1135"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017715"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0297-196"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.30.18068"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.7.2249"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.13.6721"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.4049\/jimmunol.156.3.1166","article-title":"A20 zinc finger protein inhibits TNF and IL-1 signaling","volume":"156","author":"J\u00e4\u00e4ttel\u00e4 M.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.21.12343"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1228704"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/41493"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01108.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00126-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.03.026"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09816"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3099"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.091793"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301863200"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031377"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02794"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.03.008"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1182364"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.09.015"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-8059com"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-008-0405-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.33.20029"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(00)01852-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200611128"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.241"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.240"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.10.009"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30602.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(99)01409-5"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/alzrt258"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2010.010327"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.763870"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-013-9377-4"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.6.2450-2462.2005"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.06.019"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.09.013"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801312200"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2567"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199510150-00008"},{"issue":"11","key":"e_1_2_7_48_1","first-page":"II200","article-title":"Overexpression of human copper\/zinc superoxide dismutase (SOD1) suppresses ischemia-reperfusion injury and subsequent development of graft coronary artery disease in murine cardiac grafts","volume":"110","author":"Tanaka M.","year":"2004","journal-title":"Circulation"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07575"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.5"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.14-258533","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.14-258533","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.14-258533","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:43:59Z","timestamp":1761691439000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.14-258533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,9]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1096\/fj.14-258533"],"URL":"https:\/\/doi.org\/10.1096\/fj.14-258533","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,9]]},"assertion":[{"value":"2014-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}