{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:44:58Z","timestamp":1792665898161,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T00:00:00Z","timestamp":1419897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI073745"],"award-info":[{"award-number":["AI073745"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI088092"],"award-info":[{"award-number":["AI088092"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>\n                    Akt signaling regulates diverse physiologies in a wide range of organisms. We examine the impact of increased Akt signaling in the fat body of 2 mosquito species, the Asian malaria mosquito\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    and the yellow fever mosquito\n                    <jats:italic>Aedes aegypti.<\/jats:italic>\n                    Overexpression of a myristoylated and active form of\n                    <jats:italic>A. stephensi<\/jats:italic>\n                    and\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    Akt in the fat body of transgenic mosquitoes led to activation of the downstream signaling molecules forkhead box O (FOXO) and p70 S6 kinase in a tissue and blood meal\u2010specific manner. In both species, increased Akt signaling in the fat body after blood feeding significantly increased adult survivorship relative to nontransgenic sibling controls. In\n                    <jats:italic>A. stephensi<\/jats:italic>\n                    , survivorship was increased by 15% to 45%, while in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    , it increased 14% to 47%. Transgenic mosquitoes fed only sugar, and thus not expressing active Akt, had no significant difference in survivorship relative to nontransgenic siblings. Expression of active Akt also increased expression of fat body vitellogenin, but the number of viable eggs did not differ significantly between transgenic and nontransgenic controls. This work demonstrates a novel mechanism of enhanced survivorship through increased Akt signaling in the fat bodies of multiple mosquito genera and provides new tools to unlock the molecular underpinnings of aging in eukaryotic organisms.\u2014Arik, A. J., Hun, L. V., Quicke, K., Piatt, M., Ziegler, R., Scaraffia, P. Y., Badgandi H., Riehle, M. A. Increased Akt signaling in the mosquito fat body increases adult survivorship.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 1404\u20101413 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-261479","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T22:17:48Z","timestamp":1419977868000},"page":"1404-1413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Increased Akt signaling in the mosquito fat body increases adult survivorship"],"prefix":"10.1096","volume":"29","author":[{"given":"Anam J.","family":"Arik","sequence":"first","affiliation":[{"name":"Department of Entomology University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis V.","family":"Hun","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra","family":"Quicke","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Emory University Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Piatt","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Ziegler","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia Y.","family":"Scaraffia","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine Vector\u2010Borne Infectious Diseases Research Center School of Public Health and Tropical Medicine Tulane University New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemant","family":"Badgandi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology University of Texas Southwestern Medical Center at Dallas Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Riehle","sequence":"additional","affiliation":[{"name":"Department of Entomology University of Arizona Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384749-2.10002-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.07.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.12.004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.5.1.14080"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030057"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.3.1.7771"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172539"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000857"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405775102"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02549"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1098219"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.09.013"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/366461a0"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/40194"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1476"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1078223"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9261com"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016144"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.012955"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.05.006"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085356"},{"key":"e_1_2_7_24_1","volume-title":"The Biology of Mosquitoes.","author":"Clements A. 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