{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:44:35Z","timestamp":1765485875046,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T00:00:00Z","timestamp":1424390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Catalytic antibodies have exhibited interesting functions against some infections viruses such as HIV, rabies virus, and influenza virus\n                    <jats:italic>in vitro<\/jats:italic>\n                    as well as\n                    <jats:italic>in vivo.<\/jats:italic>\n                    In some cases, a catalytic antibody light chain takes on several structures from the standpoint of molecular size (monomer, dimer, etc.) and\/or isoelectronic point. In this study, we prepared a monomeric 23D4 light chain by mutating the C\u2010terminal Cys to Ala of the wild\u2010type. The mutated 23D4 molecule took a simple monomeric form, which could hydrolyze synthetic 4\u2010methyl\u2010coumaryl\u20107\u2010amide substrates and a plasmid DNA. Because the monomeric 23D4 light chain suppressed the infection of influenza virus A\/Hiroshima\/37\/2001 in an\n                    <jats:italic>in vitro<\/jats:italic>\n                    assay, the corresponding experiments were conducted\n                    <jats:italic>in vivo<\/jats:italic>\n                    , after the virus strain (which was taken from a human patient) was successfully adapted into BALB\/cN Sea mice. In the experiments, a mixture of the monomeric 23D4 and the virus was nasally administered 1) with preincubation and 2) without preincubation. As a result, the monomeric 23D4 clearly exhibited the ability to suppress the influenza virus infection in both cases, indicating a potential drug for preventing infection of the influenza A virus.\u2014 Hifumi, E., Arakawa, M., Matsumoto, S., Yamamoto, T., Katayama, Y., Uda, T. Biochemical features and antiviral activity of a monomeric catalytic antibody light\u2010chain 23D4 against influenzaAvirus.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    29, 2347\u20102358 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-264275","type":"journal-article","created":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T03:44:00Z","timestamp":1424922240000},"page":"2347-2358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Biochemical features and antiviral activity of a monomeric catalytic antibody light\u2010chain 23D4 against influenza A virus"],"prefix":"10.1096","volume":"29","author":[{"given":"Emi","family":"Hifumi","sequence":"first","affiliation":[{"name":"Research Promotion Institute Oita 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