{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:37:32Z","timestamp":1792705052602,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T00:00:00Z","timestamp":1427241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001081","name":"Hunter Medical Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The dynamin family of GTPases has been implicated as novel regulators of the acrosome reaction, a unique exocytotic event that is essential for fertilization. Dynamin activity during the acrosome reaction is accompanied by phosphorylation of key serine residues. We now tested the hypothesis that glycogen synthase kinase 3 (GSK3) is the protein kinase responsible for dynamin phosphorylation at these phosphosites in mouse spermatozoa. Pharmacologic inhibition of GSK3 in mature mouse spermatozoa (CHIR99021: IC\n                    <jats:sub>50<\/jats:sub>\n                    = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), acrosomal exocytosis (9.7% vs. 25.7%;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01), and\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization (53% vs. 100%; P &lt; 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri\u2010acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract,\n                    <jats:italic>via<\/jats:italic>\n                    a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.\u2014Reid, A.T., Anderson, A.L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa\n                    <jats:italic>via<\/jats:italic>\n                    dynamin phosphorylation.\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 29, 2872\u20102882 (2015).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-265553","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T04:43:53Z","timestamp":1427345033000},"page":"2872-2882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa\n                    <i>via<\/i>\n                    dynamin phosphorylation"],"prefix":"10.1096","volume":"29","author":[{"given":"Andrew T.","family":"Reid","sequence":"first","affiliation":[{"name":"School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun D.","family":"Roman","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eileen A.","family":"McLaughlin","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"McCluskey","sequence":"additional","affiliation":[{"name":"School of Environmental and Life Sciences, Discipline of Chemistry, The University of Newcastle Callaghan New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip J.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Children's Medical Research Institute, The University of Sydney Westmead New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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